文献类型： 外文期刊

作者： Cao, L. J. ¹ ; Wen, J. B. ¹ ; Wei, S. J. ² ; Liu, J. ¹ ; Yang, F. ¹ ; Chen, M. ¹ ;

作者机构： 1.Beijing Forestry Univ, Coll Forestry, Beijing Key Lab Forest Pest Control, Beijing 100083, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

关键词： Lepidoptera;Hyphantria cunea;microsatellite;mutation pattern;population differentiation

期刊名称：BULLETIN OF ENTOMOLOGICAL RESEARCH （ 影响因子：1.75； 五年影响因子：2.007 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： This is the first report of microsatellite markers (simple sequence repeats, SSR) for fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae), an important quarantine pest in some European and Asian countries. Here, we developed 48 microsatellite markers for H. cunea from SSR enrichment libraries. Sequences isolated from libraries were sorted into four categories and analyzed. Our results suggest that sequences classified as Grouped should not be used for microsatellite primer design. The genetic diversity of microsatellite loci was assessed in 72 individuals from three populations. The number of alleles per locus ranged from 2 to 5 with an average of 3. The observed and expected heterozygosities of loci ranged from 0 to 0.958 and 0 to 0.773, respectively. A total of 18 out of 153 locus/population combinations deviated significantly from Hardy-Weinberg equilibrium. Moreover, significant linkage disequilibrium was detected in one pair of loci (1275 pairs in total). In the neutral test, two loci were grouped into the candidate category for positive selection and the remainder into the neutral category. In addition, a complex mutation pattern was observed for these loci, and F-ST performed better than did R-ST for the estimation of population differentiation in different mutation patterns. The results of the present study can be used for population genetic studies of H. cunea.