文献类型： 外文期刊
作者： Chang, S. 1 ; Wei, F. 3 ; Yang, Y. 3 ; Wang, A. 1 ; Jin, Z. 1 ; Li, J. 1 ; He, Y. 1 ; Shu, H. 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Haikou Expt Stn, Haikou, Peoples R China
2.Key Lab Hainan Banana Genet & Breeding, Haikou, Peoples R China
3.Zhengzhou Univ, Dept Biol, Zhengzhou 450052, Peoples R China
关键词： Phytoremediation;Mercury;Vacuole;Cytoplasm;Chelatin
期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Tobacco is an ideal plant for modification to remove mercury from soil. Although several transgenic tobacco strains have been developed, they either release elemental mercury directly into the air or are only capable of accumulating small quantities of mercury. In this study, we constructed two transgenic tobacco lines: Ntk-7 (a tobacco plant transformed with merT-merP-merB1-merB2-ppk) and Ntp-36 (tobacco transformed with merT-merP-merB1-merB2-pcs1). The genes merT, merP, merB1, and merB2 were obtained from the well-known mercury-resistant bacterium Pseudomonas K-62. Ppk is a gene that encodes polyphosphate kinase, a key enzyme for synthesizing polyphosphate in Enterobacter aerogenes. Pcs1 is a tobacco gene that encodes phytochelatin synthase, which is the key enzyme for phytochelatin synthesis. The genes were linked with LP4/2A, a sequence that encodes a well-known linker peptide. The results demonstrate that all foreign genes can be abundantly expressed. The mercury resistance of Ntk-7 and Ntp-36 was much higher than that of the wild type whether tested with organic mercury or with mercuric ions. The transformed plants can accumulate significantly more mercury than the wild type, and Ntp-36 can accumulate more mercury from soil than Ntk-7. In mercury-polluted soil, the mercury content in Ntp-36's root can reach up to 251 mu g/g. This is the first report to indicate that engineered tobacco can not only accumulate mercury from soil but also retain this mercury within the plant. Ntp-36 has good prospects for application in bioremediation for mercury pollution.
- 相关文献
作者其他论文 更多>>
-
Effect of lipids on the stability of natural rubber latex and tensile properties of its films (vol 20, pg 213, 2017)
作者：Liu, H.;Wang, Q.;Li, J.;Liu, Y.;He, D.;Yu, H.;Gao, X.;Li, J.;Liu, Y.;He, D.
关键词：
-
Comparison of Microbial Communities in the Rhizosphere Soils from Two Varieties of Camellia sinensis in Yunnan Province, China
作者：Yang, Y.;Wu, H.;Wang, Sh
关键词：Camellia sinensis; medium-small-leaf variety; small-leaf variety; microbiome; rhizosphere soil
-
Multiplex RT-PCR to simultaneously detect three viruses that infect peach
作者：Xu, Y.;Yang, L.;Zhou, J.;Lu, M.;Li, S.;Yang, Y.;Li, S.
关键词：multiplex RT-PCR; peach; peach-associated luteovirus; peach virus D; nectarine stem-pitting-associated virus; peach
-
Effect of Lipids on the Stability of Natural Rubber Latex and Tensile Properties of its Films
作者：Yu, H.;Wang, Q.;Li, J.;Liu, Y.;He, D.;Yu, H.;Gao, X.;Li, J.;Liu, Y.;He, D.
关键词：Natural rubber latex; phospholipids; gas chromatography-mass spectrometry; mechanical stability; tensile properties
-
Effect of Continuous Cropping on Soil Chemical Properties and Crop Yield in Banana Plantation
作者：Zhong, S.;Guo, G.;Zeng, H.;Jin, Z.;Zhong, S.;Mo, Y.
关键词：Balanced fertilization;Continuous cropping;Latosols;Nutrients accumulation;Soil acidity;Soil quality;Sustainability
-
A method to suppress the browning in banana (Musa, AAA) embryogenic callus induced
作者：Chang, S.;Shu, H.;Chang, S.;Shu, H.;Chang, S.
关键词：Banana;browning;embryogenic callus;embryo genic cell suspension
-
Skin Pigmentation Evaluation in Broilers Fed Different Levels of Natural Okra and Synthetic Pigments
作者：Liu, G. -D.;Hou, G. -Y.;Wang, D. -J.;Zhang, X. -Y.;Sun, W. -P.;Lv, S. -J.;Yang, Y.
关键词：poultry pigmentation;okra;xanthophyll;Da-Ma-Hua;color evaluation
