您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

An efficient PEG-mediated transient gene expression system in grape protoplasts and its application in subcellular localization studies of flavonoids biosynthesis enzymes

文献类型： 外文期刊

作者： Wang, Huiling 1 ; Wang, Wei 4 ; Zhan, Jicheng 2 ; Huang, Weidong 2 ; Xu, Haiying 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 100097, Peoples R China

2.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

3.Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing, Peoples R China

4.Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

关键词： Transient expression;Protoplast;Grape;Subcellular localization;Flavonoid

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Transient gene expression utilizing plant protoplasts has emerged as a refined method to identify proteins function in vivo. In this study, an improved protocol for preparation of protoplasts from grape berry (cabernet sauvignon) suspension cells and an optimized system for polyethylene glycol (PEG)-mediated transient transformation of the protoplasts with the application of the green fluorescent protein (GFP) as a reporter gene was presented. Step by step optimization showed that the quality of protoplasts as well as PEG-mediated transformation conditions including amount of plasmid DNA, the PEG concentration, transfection duration and the status of suspension cells used for protoplasts preparation were the major factors which influenced successful transient gene expression assays. Following the optimized protocol, up to half of the transfected protoplasts displayed green fluorescence in the total observed cells. To demonstrate the applicability of the system, the subcellular localization of VvCHS, VvCHI, VvUEGT and VvANR were investigated, revealing the cytoplasm and nucleus localization of flavonoids biosynthesis enzymes in grape. This improved transient gene expression system in grape protoplasts gives a rapid and efficient alternative for future research in grapevine molecular biology. (c) 2015 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Expression and tissue and subcellular localization of anthocyanidin synthase (ANS) in grapevine. Wang, Huiling,Wang, Wei,Li, Hui,Zhang, Ping,Zhan, Jicheng,Huang, Weidong,Wang, Wei.

[2]Identification of fungus-responsive cis-acting element in the promoter of Brassica juncea chitinase gene, BjCHI1. Gao, Ying,Zan, Xin-Li,Wu, Xue-Feng,Jia, Shuang-Wei,Zhao, Kai-Jun,Zan, Xin-Li,Chen, Yu-Ling,Yao, Lei. 2014

[3]Research on Grape Deterioration Process via Volatiles-Using Long Optical-Path Infrared Spectroscopy and Simplified E-Nose. Zheng Wen-gang,Jiao Lei-zi,Zhao Xian-de,Dong Da-ming. 2016

[4]Analysis and discrimination of grape spoilage via volatiles: a comparison between long optical path Fourier-transform-infrared spectroscopy and sensor arrays. Dong, D.,Zheng, W.,Wang, W.,Zhao, X.,Jiao, L.,Zhao, C..

[5]Determination of oxathiapiprolin concentration and dissipation in grapes and soil by ultrahigh-performance liquid chromatography-tandem mass spectrometry. Yu, Pingzhong,Jia, Chunhong,Zhao, Ercheng,Chen, Li,Jing, Junjie,He, Min,He, Hongju.

[6]Molecular cloning, expression analysis, and subcellular localization of FLOWERING LOCUS T (FT) in carrot (Daucus carota L.). Zhan, Zongxiang,Zhang, Chunsha,Zhang, Hongwei,Li, Xiaojie,Wen, Changlong,Liang, Yi,Zhan, Zongxiang,Zhan, Zongxiang. 2017

[7]Bioinformatic Analyses of Subgroup-A Members of the Wheat bZIP Transcription Factor Family and Functional Identification of TabZIP174 Involved in Drought Stress Response. Li, Xueyin,Ma, Lingjian,Feng, Biane,Zhang, Fengjie,Tang, Yimiao,Zhang, Liping,Zhao, Changping,Gao, Shiqing,Feng, Biane,Zhang, Fengjie. 2016

[8]Subcellular localization of endogenous IAA during poplar leaf rhizogenesis revealed by in situ immunocytochemistry. Dong, Ningguang,Hao, Yanbin,Dong, Ningguang,Gao, Ying,Pei, Dong,Dong, Ningguang,Yin, Weilun.

[9]Molecular cloning, expression analysis and subcellular localization of LEAFY in carrot (Daucus carota L.). Zhang, Chunsha,Liang, Yi,Zhang, Hongwei,Zhan, Zongxiang,Zhan, Zongxiang.

[10]Gene transcript accumulation, tissue and subcellular localization of anthocyanidin synthase (ANS) in developing grape berries. Wang, Huiling,Wang, Wei,Zhang, Ping,Pan, QiuHong,Zhan, Jicheng,Huang, Weidong,Wang, Wei.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台