文献类型： 外文期刊
作者： Chang, Y. Y. 1 ; Ji, X. 1 ; Zhu, J. L. 2 ; Hao, Y. 3 ;
作者机构： 1.Gansu Agr Univ, Coll Agron Sci, Lanzhou, Peoples R China
2.Lanzhou Agr Univ, Coll Grassland Sci, Lanzhou, Peoples R China
3.Gansu Acad Agr Sci, Inst Fruit & Floriculture Res, Lanzhou, Peoples R China
关键词： chromosome;doubling rate
期刊名称：X INTERNATIONAL CONFERENCE ON GRAPEVINE BREEDING AND GENETICS
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收录情况： SCI
摘要： Stem segments of the tissue culture-grown seedlings of the Victoria grape were soaked 24 or 48 h using an aqueous solution of colchicine (0.05% or 0.1%). The best treatment for chromosome duplication efficiency was the use of the third and fourth budstreated for 48 h in 0.05% colchicine or 24 h in 0.1% colchicine. The doubling rates of primary generation cells were 33% and 31%; respectively. The polyploid cell percentage increases about 10% by subculture of the chimeras. The polyploid cell percentage could reach 75.8%-96.6% by subculturing the chimeras for four to six generations. Through root tip cell chromosome observations, there was little effect on the division cycle of root-tip cells in terms of the cultured seedlings with or without illumination in the short term, and the optimal sampling time of root tips for chromosome observation was at 9:30 am.
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