Efficient Inoculation of Rice black-streaked dwarf virus to Maize Using Laodelphax striatellus Fallen
文献类型: 外文期刊
作者: Miao, Hong-Qin 1 ; Di, Dian-Ping 1 ; Zhang, Ai-Hong 1 ; Lu, Yin-Gui 1 ; Tian, Lan-Zhi 1 ; Stewart, Lucy R. 2 ; Redinba 1 ;
作者机构: 1.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Baoding 071000, Hebei, Peoples R China
2.Ohio State Univ, Dept Plant Pathol, Ohio Agr Res & Dev Ctr, USDA ARS Corn Soybean & Wheat Qual Res, Wooster, OH 44691 USA
关键词: inoculation technique;Laodelphax striatellus Fallen;maize;Rice black-streaked dwarf virus
期刊名称:JOURNAL OF PHYTOPATHOLOGY ( 影响因子:1.789; 五年影响因子:1.574 )
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收录情况: SCI
摘要: Maize rough dwarf disease caused by Rice black-streaked dwarf virus (RBSDV) is the most important disease of maize in China. Although deploying disease resistant hybrids would be the most effective way to control the disease, development of resistant hybrids has been limited by virus transmission rates that are too low for effective screening. An efficient inoculation technique for RBSDV was developed using Laodelphax striatellus Fallen, in which a virus-free planthopper colony was developed and viruliferous planthoppers were obtained by allowing a 3- to 4-day acquisition access period on RBSDV-infected wheat plants. Planthoppers were then allowed a 25- to 28-day latent period on wheat seedlings followed by a 3-day inoculation access period on two-to-three-leaf stage maize seedlings. By 35days postinoculation, susceptible hybrid Zhengdan 958', inbred lines of Ye 107' and Ye 478' plants showed 100% RBSDV infection with symptoms of stunting plants, darkening leaves and white waxy swellings on underside of leaves. At tasseling stage, average disease indices were from 96.4 to 100.0%. Enzyme-linked immunosorbent assays were correlated with the presence of symptoms. The high efficiency of RBSDV transmission obtained using this technique provides a reliable procedure to screen for RBSDV resistance in maize.
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