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DegQ regulates the production of fengycins and biofilm formation of the biocontrol agent Bacillus subtilis NCD-2

文献类型： 外文期刊

作者： Wang, Peipei 1 ; Guo, Qinggang 2 ; Ma, Yinan; Li, Shezeng 2 ; Lu, Xiuyun 2 ; Zhang, Xiaoyun 3 ; Ma, Ping;

作者机构： 1.Agr Univ Hebei, Coll Plant Protect, Baoding 071000, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Integrated Pest Management Ctr Hebei Prov, Key Lab IPM Crops Northern Reg North China,Minist, Baoding City 071000, Hebei Province, Peoples R China

3.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Integrated Pest Management Ctr Hebei Prov, Key Lab IPM Crops Northern Reg North China,Minist, Baoding City 0

关键词： Regulator;Mutagenicity;Lipopeptides;Botrytis cinerea

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：5.415； 五年影响因子：6.038 ）

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收录情况： SCI

摘要： Bacillus subtilis NCD-2 is an excellent biocontrol agent for tomato gray mold and cotton soil-borne diseases. The fengycin lipopeptides serve as a major role in its biocontrol ability. A previous study revealed that insertion of degQwith the mini-Tn10 transposon decreased the antifungal activity of strain NCD-2 against the growth of Botrytis dnerea. To clarify the regulation of degQ on the production of fengycin, we deleted degQ by in-frame mutagenesis. Compared with the wild-type strain NCD-2, the degQ-null mutant had decreased extracellular protease and cellulase activities as well as antifungal ability against the growth of B. cinerea in vitro. The lipopeptides from the degQ-null mutant also had significantly decreased antifungal activity against B. cinerea in vitro and in vivo. This result was confirmed by the decreased fengycin production in the degQ-null mutant that was detected by fast protein liquid chromatography analysis. Quantitative reverse transcription PCR further demonstrated that degQ positively regulated the expression of the fengycin synthetase gene. In addition, the degQ-null mutant also had a flatter colony phenotype and significantly decreased biofilm formation ability relative to the wild-type strain. All of those characteristics from degQ-null mutant could be restored to the strain NCD-2 wild-type level by complementation of intact degQ in the mutant. Therefore, DegQ may be an important regulator of fengycin production and biofilm formation in B. subtilis NCD-2. (C) 2015 Elsevier GmbH. All rights reserved.

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[1]Fengycin produced by Bacillus subtilis NCD-2 plays a major role in biocontrol of cotton seedling damping-off disease. Guo, Qinggang,Dong, Weixin,Li, Shezeng,Lu, Xiuyun,Wang, Peipei,Zhang, Xiaoyun,Ma, Ping,Dong, Weixin,Wang, Ye.

[2]The PhoR/PhoP two-component system regulates fengycin production in Bacillus subtilis NCD-2 under low-phosphate conditions. Guo Qing-gang,Dong Li-hong,Wang Pei-pei,Li She-zeng,Zhao Wei-song,Lu Xiu-yun,Zhang Xiao-yun,Ma Ping. 2018

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