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Construction and evaluation of a transformant library of Lasiodiplodia theobromae generated through Restriction Enzyme-Mediated Integration

文献类型： 外文期刊

作者： Zhang, Wei 1 ; Liu, Aifen 1 ; Zhang, Xin 1 ; Yan, Hong 1 ; Liu, Ruiqi 1 ; Pang, Qianru 1 ; Huang, Jinguang 2 ; Li, Xingho 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

2.Qingdao Agr Univ, Coll Agr & Plant Protect, Qingdao 266109, Peoples R China

3.Agr Univ Hebei, Coll Life Sci, Baoding 071001, Peoples R China

4.Agr Univ Hebei, Coll Life Sci,

关键词： mutants;grapevine Botryosphaeria dieback;pathogenicity variant

期刊名称：PHYTOPATHOLOGIA MEDITERRANEA （ 影响因子：2.02； 五年影响因子：2.08 ）

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收录情况： SCI

摘要： Grapevine dieback, caused by Lasiodiplodia theobromae, is an important trunk disease worldwide. Transformants of L. theobromae were generated in an attempt to identify potential pathogenicity-related genes. Lasiodiplodia theobromae strain JZB 0300251, a highly virulent isolate, was selected for the genetic transformation. Based on optimised conditions, the Restriction Enzyme-Mediated Integration (REMI) methodology was established in L. theobromae using pUCATPH (a plasmid carrying a hygromycin B marker). A total of 6,036 transformants were generated with four restriction enzymes, respectively and the transformant library was evaluated based on 200 randomly selected transformants. Mutants that exhibited various degrees of virulence and different growth rates were obtained. The study provides basic results that will lead to increased understanding of the role of the pathogenicity-related genes involved in the infection process of L. theobromae.

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