Construction and evaluation of a transformant library of Lasiodiplodia theobromae generated through Restriction Enzyme-Mediated Integration
文献类型： 外文期刊
作者： Zhang, Wei 1 ; Liu, Aifen 1 ; Zhang, Xin 1 ; Yan, Hong 1 ; Liu, Ruiqi 1 ; Pang, Qianru 1 ; Huang, Jinguang 2 ; Li, Xingho 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China
2.Qingdao Agr Univ, Coll Agr & Plant Protect, Qingdao 266109, Peoples R China
3.Agr Univ Hebei, Coll Life Sci, Baoding 071001, Peoples R China
4.Agr Univ Hebei, Coll Life Sci,
关键词： mutants;grapevine Botryosphaeria dieback;pathogenicity variant
期刊名称：PHYTOPATHOLOGIA MEDITERRANEA （ 影响因子：2.02； 五年影响因子：2.08 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Grapevine dieback, caused by Lasiodiplodia theobromae, is an important trunk disease worldwide. Transformants of L. theobromae were generated in an attempt to identify potential pathogenicity-related genes. Lasiodiplodia theobromae strain JZB 0300251, a highly virulent isolate, was selected for the genetic transformation. Based on optimised conditions, the Restriction Enzyme-Mediated Integration (REMI) methodology was established in L. theobromae using pUCATPH (a plasmid carrying a hygromycin B marker). A total of 6,036 transformants were generated with four restriction enzymes, respectively and the transformant library was evaluated based on 200 randomly selected transformants. Mutants that exhibited various degrees of virulence and different growth rates were obtained. The study provides basic results that will lead to increased understanding of the role of the pathogenicity-related genes involved in the infection process of L. theobromae.
- 相关文献
作者其他论文 更多>>
-
An Original UV Adhesive Watermelon Grafting Method, the Grafting Device, and Experimental Verification
作者：Zhang, Xin;Kong, Linghao;Lu, Hanwei;Zhang, Xin;Feng, Qingchun;Li, Tao;Jiang, Kai;Zhang, Qian
关键词：watermelon grafting; UV adhesive; fluent; VOF-DPM numerical simulation; grafting device; test
-
Insights into the mechanism of L-malic acid on drip loss of chicken meat under commercial conditions
作者：Sun, Haijun;Wang, Lu;Zhu, Ruimin;Yin, Jingdong;Zhang, Xin;Yan, Xue;Chen, Meixia
关键词：Drip loss; Immune response; L-malic acid; Meat quality; Metabolome; Transcriptome
-
Maternal Malic Acid May Ameliorate Oxidative Stress and Inflammation in Sows through Modulating Gut Microbiota and Host Metabolic Profiles during Late Pregnancy
作者：Chen, Meixia;Liu, Hui;Zhang, Dongyan;Wang, Sixin;Wang, Jing;Zhao, Ying;Li, Shuang;Chang, Zhuo;Zhang, Xin
关键词：L-malic acid; late pregnancy; sow; gut microbiota; metabolic
-
The maternal lifestyle in pregnancy: Implications for foetal skeletal muscle development
作者：Sun, Haijun;Liao, Jialong;He, Linjuan;Wan, Boyang;Yin, Jingdong;Zhang, Xin;Chen, Meixia;Yin, Jingdong;Zhang, Xin
关键词：foetal skeletal muscle development; gut microbiota; maternal exercise; maternal nutrition; metabolic disorders
-
Study on the Short-Term Preservation of Gametes, Cross-Stimulation of Oocytes by Distant Sperm, and the Impact of Cold-Stimulated Fertilized Eggs on Eyes in the Celestial Goldfish
作者：Li, Rongni;Sun, Yansheng;Zhang, Xin;Li, Wentong
关键词：celestial rate of goldfish; gamete preservation; distant hybridization; pre-fertilization treatment; ploidy analysis
-
Study on the Effects of Different Light Supply Modes on the Development and Extracellular Enzyme Activity of Ganoderma lucidum
作者：Liu, Yihan;Luo, Yuan;Guo, Wenzhong;Zhang, Xin;Chen, Xiaoli;Liu, Yihan;Zheng, Wengang
关键词：edible fungi; Ganoderma lucidum; light supply mode; nutritional quality; extracellular enzyme
-
Chenodeoxycholic Acid Improves Embryo Implantation and Metabolic Health through Modulating Gut Microbiota-Host Metabolites Interaction during Early Pregnancy
作者：Chen, Meixia;Ji, Haifeng;Li, Lu;Liu, Hui;Wang, Sixin;Zhang, Dongyan;Wang, Jing;Zhao, Ying;Li, Lu;Yin, Jingdong;Zhang, Xin
关键词：chenodeoxycholic acid; early pregnancy; embryo implantation; gut microbiota; metabolic health; metabolites
