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Expression of Paenibacillus polymyxa beta-1,3-1,4-glucanase in Streptomyces lydicus A01 improves its biocontrol effect against Botrytis cinerea

文献类型： 外文期刊

作者： Li, Jinjin 1 ; Liu, Weicheng 1 ; Luo, Lijin 2 ; Dong, Dan 1 ; Liu, Ting 1 ; Zhang, Taotao 1 ; Lu, Caige 1 ; Liu, Dewen 1 ; Z 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

2.Fujian Inst Microbiol, Fujian Prov Key Lab Screening Novel Microbial Pro, Fuzhou 350007, Peoples R China

关键词： beta-1;3-1;4-Glucanase;Antifungal activity;Paenibacillus polymyxa;Streptomyces lydicus;Natamycin

期刊名称：BIOLOGICAL CONTROL （ 影响因子：3.687； 五年影响因子：3.962 ）

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收录情况： SCI

摘要： Streptomyces lydicus strain A01, which can produce natamycin and chitinase, has a significant inhibition effect on gray mold disease caused by Botrytis cinerea. However, it has no detectable glucanase activity. Strain A21 isolated from the snow covered high altitude area in Tibet, China, also has a high antagonistic activity against B. cinerea. It displayed an obvious halo on lichen polysaccharides plates by congo red staining, indicating a strong glucanase activity. A21 was identified as Paenibacillus polymyxa using 16S rDNA gene analysis and biochemical and physiological analysis. To obtain the synergistic antifungal effects of natamycin, chitinase, and glucanases on B. cinerea, this study transformed the beta-1,3-1,4-glucanase gene from P. polymyxa A21 to S. lydicus A01. The engineered S. lydicus AG01 showed substantially high glucanase activity, and had similar natamycin production and chitinase activity as the wild-type strain A01. Compared to the wild-type strain A01, the antifungal effects of S. lydicus AG01 on B. cinerea, including inhibition of spore germination and mycelial growth, were highly improved. The improved biocontrol effect of S. lydicus AG01 is likely attributed to the heterologous expression of glucanase from P. polymyxa, which acted synergistically with natamycin and chitinase to increase the antifungal activity of the strain. (C) 2015 The Authors. Published by Elsevier Inc.

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[1]IDENTIFICATION OF AN ANTIFUNGAL METABOLITE PRODUCED BY A POTENTIAL BIOCONTROL ACTINOMYCES STRAIN A01. Lu, Cai Ge,Liu, Wei Cheng,Qiu, Ji Yan,Liu, Ting,Liu, De Wen,Lu, Cai Ge,Wang, Hui Min. 2008

[2]Optimization of the Fermentation Process for Streptomyces lydicus A02 Producing Natamycin. Weicheng Liu,Ting Liu,Caige Lu,Taotao Zhang,Dewen Liu,Yupeng Hua. 2012

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