您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Mining of genic SNPs and diversity evaluation of landraces in loquat

文献类型： 外文期刊

作者： Li, Xiaoying 1 ; Xu, Hongxia 1 ; Feng, Jianjun 2 ; Zhou, Xiaoyin 3 ; Chen, Junwei 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China

2.Ninghai Agr & Forestry Bur, Ningbo 315600, Zhejiang, Peoples R China

3.Lishui Agr Bur, Lishui 323000, Zhejiang, Peoples R China

关键词： Loquat;Genic SNPs;Characteristic analysis;Germplasm evaluation;Phylogenetic tree

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： With the development of next-generation sequencing technologies, the exploitation of single nucleotide polymorphism (SNP) markers from transcriptome sequences has gained more attention and this kind of marker is widely utilized in plant genetic research. In this study, genic SNP markers derived from transcriptome sequences were identified from 26 loquat cultivars. A total length of 4183 bp using 9 sets of primers was sequenced and used for alignments. From this, 103 SNPs, with a frequency of one SNP per 41 bp, were found, which were comprised of 55 transitions, 37 transversions, 8 InDels and 3 other types. Among these SNPs, the percentage of transitions and transversions in A/G, C/T, G/T, A/T, G/C and A/C were 31.1% (32), 22.3% (23), 13.6% (14), 11.7% (12), 6.8%(7) and 3.9% (4), respectively. Of these SNP genotypes, 84 were detected as homozygotes in the 26 loquat cultivars, and 8 heterozygous SNPs were found in some cultivars. Cluster analysis of the 26 loquat cultivars was also carried out using the SNPs data, and a phylogenetic tree constructed showed that all the cultivars were separated well, and clustered into several groups which basically corresponds to the genetic background of them. The newly identified genic SNPs in this study will provide powerful tools in molecular studies for cultivar identification, genetic diversity analyses and marker-assisted selection breeding in loquat (C) 2015 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Genetic diversity and relationships among 47 loquat varieties revealed by EST-SSR markers. Li, Xiao-ying,Xu, Hong-xia,Chen, Jun-wei. 2013

[2]Seasonal variations in the contents of nutrient elements in the leaves and the fruits of Eriobotry japonica L. Ding, CK,Chen, QF,Sun, TL. 1995

[3]Rapid identification of red-flesh loquat cultivars using EST-SSR markers based on manual cultivar identification diagram strategy. Li, X. Y.,Xu, H. X.,Chen, J. W.. 2014

[4]Effect of hypobaric storage on physiological and quality attributes of loquat fruit at low temperature. Gao, H. Y.,Chen, H. J.,Chen, W. X.,Yang, J. T.,Song, L. L.,Zheng, Y. H.,Jiang, Y. M.. 2006

[5]Comparison of phenolic compound contents and antioxidant capacities of loquat (Eriobotrya japonica Lindl.) fruits. Xu, Hong-xia,Li, Xiao-ying,Chen, Jun-wei. 2014

[6]Comparative transcriptome profiling of freezing stress responses in loquat (Eriobotrya japonica) fruitlets. Xu, Hong-xia,Li, Xiao-ying,Chen, Jun-wei.

[7]Overexpression of a loquat (Eriobotrya japonica Lindl.) vacuolar invertase affects sucrose levels and growth. Wang, Yanpeng,Qin, Qiaoping,Chen, Junwei,Feng, Jianjun,Huang, Jianqin.

[8]Variation between Turnip mosaic virus isolates in Zhejiang Province, China and evidence for recombination. Chen, JP,Chen, JP,Adams, MJ. 2002

[9]Molecular and biochemical characterization of manganese-containing superoxide dismutase from the silkworm, Bombyx mori. Yamamoto, K,Zhang, PB,He, NJ,Wang, YQ,Aso, Y,Banno, Y,Fujii, H. 2005

[10]Genetic diversity of the VP1 gene of duck hepatitis virus type I (DHV-I) isolates from southeast China is related to isolate attenuation. Liu, Guangqing,Ni, Zheng,Yun, Tao,Yu, Bin,Huang, Jionggang,Chen, Jianping,Wang, Fei. 2008

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台