您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Histological and endogenous plant growth regulators changes associated with adventitious shoot regeneration from in vitro leaf explants of strawberry (Fragaria x ananassa cv. 'Honeoye')

文献类型： 外文期刊

作者： Wang, Hua 1 ; Li, Maofu 1 ; Yang, Yuan 1 ; Dong, Jing 1 ; Jin, Wanmei 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing, Peoples R China

关键词： 'Honeoye';Leaf disk regeneration;Histology;Plant growth regulators;HPLC-MS/MS

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Few studies have been conducted on the links between histological and hormonal variation during shoot regeneration. Therefore, we investigated this link in strawberry (Fragaria x ananassa cv. 'Honeoye'). Main plant growth regulators were measured using reverse-phase liquid chromatography-tandem mass spectrometry. Histological observations were conducted to understand the pattern of adventitious shoot regeneration from the leaf segments. After 14 days of dark culture, a mean shoot regeneration frequency of 94.7 % was obtained on MS medium supplemented with 2.0 mg L-1 thidiazuron, 30 g L-1 sucrose, and 6 g L-1 agar (pH 5.9). During shoot regeneration, indole acetic acid (IAA) concentrations increased, abscisic acid (ABA) decreased, and gibberellic acid (GA(3)) and zeatin showed peaks. The results could be correlated with the cell division and differentiation that occurred during shoot regeneration. Histological observation showed that the adventitious shoots were derived from subepidermal cells and the epidermal cells of the midrib near the cut. The meristemoids, primordia and shoots were formed sequentially on day 6, day 12, and day 18 after culture. During the meristemoids formation on day 6 after culture, IAA rapidly increased from 1.49 to 1.72 ng g(-1) fresh weight (Fw), ABA rapidly decreased from 52.61 to 13.47 ng g(-1) Fw, and zeatin increased from 1.68 to 5.98 ng g(-1) Fw. During primordia formation on day 12 after culture, IAA rapidly increased to 1.88 ng g(-1) Fw, ABA rapidly decreased to 2.69 ng g(-1) Fw, GA(3) peaked at 73.91 ng g(-1) Fw, and zeatin peaked at 7.69 ng g(-1) Fw. Our results suggested that the histological variations were consistent with the plant hormonal changes during shoot regeneration, and that changes in hormone concentration could be used as a reference to characterize the mode of shoot regeneration.

  • 相关文献

[1]Adventitious shoot regeneration from leaves of apple rootstock 'Pingyitiancha' (Malus hupehensis var. pinyiensis) and genetic fidelity of regenerated plantlets using SSR markers. Jin, Wanmei,Wang, Yuanhua,Wang, Hua. 2014

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台