Newly identified RNAs of raspberry leaf blotch virus encoding a related group of proteins
文献类型： 外文期刊
作者： Lu, Yuwen 1 ; McGayin, Wendy 3 ; Cock, Peter J. A. 3 ; Schnettler, Esther 4 ; Yan, Fei 1 ; Chen, Jianping 1 ; MacFarla 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Key Lab Biotechnol, State Key Lab Breeding Base Sustainable Control P, Hangzhou 310021, Zhejiang, Peoples R China
2.Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China
3.James Hutton Inst, Dundee DD2 5DA, Scotland
4.MRC Univ Glasgow, Ctr Virus Res, Glasgow G61 1QH, Lanark, Scotland
期刊名称：JOURNAL OF GENERAL VIROLOGY （ 影响因子：3.891； 五年影响因子：3.719 ）
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收录情况： SCI
摘要： Members of the genus Emaravirus, including Raspberry leaf blotch virus (RLBV), are enveloped plant viruses with segmented genomes of negative-strand RNA, although the complete genome complement for any of these viruses is not yet clear. Currently, wheat mosaic virus has the largest emaravirus genome comprising eight RNAs. Previously, we identified five genomic RNAs for RLBV; here, we identify a further three RNAs (RNA6-8). RNA6-8 encode proteins that have clear homologies to one another, but not to any other eniaravirus proteins. The proteins self-interacted in yeast two-hybrid and bimolecular fluorescence complementation (BiFC) experiments, and the P8 protein interacted with the virus nucleocapsid protein (P3) using BiFC. Expression of two of the proteins (P6 and P7) using potato virus X led to an increase in virus titre and symptom severity, suggesting that these proteins may play a role in RLBV pathogenicity; however, using two different tests, RNA silencing suppression activity was not detected for any of the RLBV proteins encoded by RNA2-8.
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