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The interaction of two-spotted spider mites, Tetranychus urticae Koch, with Cry protein production and predation by Amblyseius andersoni (Chant) in Cry1Ac/Cry2Ab cotton and Cry1F maize

文献类型: 外文期刊

作者: Guo, Yan-Yan 1 ; Tian, Jun-Ce 3 ; Shi, Wang-Peng 2 ; Dong, Xue-Hui 4 ; Romeis, Joerg 5 ; Naranjo, Steven E. 6 ; Hellmi 1 ;

作者机构: 1.Cornell Univ, NYSAES, Dept Entomol, Geneva, NY USA

2.China Agr Univ, Dept Entomol, Beijing 100094, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou, Zhejiang, Peoples R China

4.China Agr Univ, Dept Agr Sci, Beijing 100094, Peoples R China

5.ISS, Agroscope, Zurich, Switzerland

6.ARS, Arid Land Agr Res Ctr, USDA, Maricopa, AZ 85138 USA

7.ARS, Corn Insects & Crop Genet Res Unit, USDA, Ames, IA 50011 USA

8.Iowa State Univ, Dept Entomol, Ames, IA 50011 USA

关键词: Tri-trophic exposure;Cry1Ac;Cry2Ab;Cry1F;Environmental risk assessment;Biological control

期刊名称:TRANSGENIC RESEARCH ( 影响因子:2.788; 五年影响因子:2.377 )

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年卷期:

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收录情况: SCI

摘要: Crops producing insecticidal crystal (Cry) proteins from the bacterium, Bacillus thuringiensis (Bt), are an important tool for managing lepidopteran pests on cotton and maize. However, the effects of these Bt crops on non-target organisms, especially natural enemies that provide biological control services, are required to be addressed in an environmental risk assessment. Amblyseius andersoni (Acari: Phytoseiidae) is a cosmopolitan predator of the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae), a significant pest of cotton and maize. Tri-trophic studies were conducted to assess the potential effects of Cry1Ac/Cry2Ab cotton and Cry1F maize on life history parameters (survival rate, development time, fecundity and egg hatching rate) of A. andersoni. We confirmed that these Bt crops have no effects on the biology of T. urticae and, in turn, that there were no differences in any of the life history parameters of A. andersoni when it fed on T. urticae feeding on Cry1Ac/Cry2Ab or non-Bt cotton and Cry1F or non-Bt maize. Use of a susceptible insect assay demonstrated that T. urticae contained biologically active Cry proteins. Cry proteins concentrations declined greatly as they moved from plants to herbivores to predators and protein concentration did not appear to be related to mite density. Free-choice experiments revealed that A. andersoni had no preference for Cry1Ac/Cry2Ab cotton or Cry1F maize-reared T. urticae compared with those reared on non-Bt cotton or maize. Collectively these results provide strong evidence that these crops can complement other integrated pest management tactics including biological control.

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