Development of Highly Polymorphic Expressed Sequence Tags-Simple Sequence Repeat Markers and Their Application in Analysis of Genetic Diversity of Chinese Bayberry (Morella rubra)
文献类型： 外文期刊
作者： Wang, Wenting 1 ; Feng, Chao 2 ; Zhang, Zehuang 3 ; Yan, Liju 4 ; Ding, Maomao 5 ; Xu, Changjie 5 ; Chen, Kunsong 5 ;
作者机构： 1.Zhejiang Univ, State Agr Minist, Lab Hort Plant Growth Dev & Qual Improvement, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China
2.Chinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China
3.Fujian Acad Agr Sci, Fruit Res Inst, Fuzhou 350013, Peoples R China
4.Linhai Tech Extens Stn Characterist Agr Prod, Linhai 317000, Peoples R China
5.Zhejiang Univ, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China
关键词： accession discrimination;RNA-Seq;PCR stutter band;slippage amplification;phylogenetic relationship;white cultivar
期刊名称：HORTSCIENCE （ 影响因子：1.455； 五年影响因子：1.617 ）
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收录情况： SCI
摘要： Chinese bayberry (Morella rubra) is an economically important subtropical evergreen fruit crop native to China and other Asian countries. For facilitating cultivar discrimination and genetic diversity analysis, a total of 38 high-quality and highly polymorphic expressed sequence tags-simple sequence repeat (EST-SSR) markers, with little or no polymerase chain reaction (PCR) stutter bands, including 21 screened from those obtained previously and 17 newly developed markers, were developed. The average number of alleles (N-a) per locus was 5.6, and polymorphism information content varied from 0.34 to 0.86, with a mean value of 0.57. With these markers, all 42 Chinese bayberry accessions analyzed were successfully discriminated and the phylogenetic relationship between accessions was revealed. The accessions can be separated into two groups with six subgroups. The grouping of four main cultivars in three subgroups and 12 white-fruited accessions, each with little or no anthocyanin accumulation in ripe fruit, into five subgroups suggested the preservation of broad diversity among cultivated populations. These EST-SSR markers and the findings obtained in this study can assist the discrimination of cultivars and lines and contribute to genetic and breeding studies in Chinese bayberry.
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