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Functional analysis of the G-protein alpha subunits FGA1 and FGA3 in the banana pathogen Fusarium oxysporum f. sp cubense

文献类型: 外文期刊

作者: Guo, Lijia 1 ; Yang, Yuhua 1 ; Yang, Laying 1 ; Wang, Feiyang 1 ; Wang, Guofen 1 ; Huang, Junsheng 1 ;

作者机构: 1.Minist Agr, Key Lab Integrated Pest Management Trop Crops, Beijing, Peoples R China

2.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Hainan 571101, Peoples R China

3.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Hainan

关键词: Fusarium oxysporum f. sp cubense;Musa spp.;Pathogenicity;Development;Cyclic AMP

期刊名称:PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY ( 影响因子:2.747; 五年影响因子:2.388 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The asexual fungus Fusarium oxysporum f. sp. cubense (Foc) is the causal agent of fusarium wilt in bananas (Musa spp.). This fungus poses a threat to banana production throughout the world. Here, two Foc genes, fga1 and fga3, were functionally characterized. These genes encode proteins homologous to the G-protein alpha subunits GPA1 from Saccharomyces cerevisiae and MAGC from Magnaporthe grisea, respectively. The deletion of fga1 leads to a phenotypic defect in colony morphology and reductions in vegetative growth, conidiation and pathogenicity against the banana plant (Musa spp. cv. Brazil), which was not observed for the Delta fga3 deletion mutant. Intriguingly, both Delta fga1 and Delta fga3 deletion mutants showed declines in intracellular cyclic AMP levels and increases in heat resistance, suggesting that FGA1 regulates growth, development, pathogenicity, and heat resistance, whereas FGA3 modulates heat resistance, potentially through the cAMP-dependent protein kinase A pathway. These findings offer insights into the roles of the G-protein alpha subunits in the development and pathogenicity of the fungus Foc. (C) 2016 Elsevier Ltd. All rights reserved.

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