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Detection and quantification of airborne inoculum of Blumeria graminis f. sp. tritici using quantitative PCR

文献类型： 外文期刊

作者： Cao, Xueren 1 ; Yao, Dongming 1 ; Zhou, Yilin 1 ; West, Jonathan S. 3 ; Xu, Xiangming 4 ; Luo, Yong 5 ; Ding, Kejian 2 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.Anhui Agr Univ, Coll Plant Protect, Hefei 230036, Peoples R China

3.Rothamsted Res, Harpenden AL5 2JQ, Herts, England

4.East Malling Res, New Rd, East Malling ME19 6BJ, Kent, England

5.China Agr Univ, Dept Plant

关键词： Blumeria graminis f. sp tritici;Wheat powdery mildew;Real-time PCR;Airborne conidia;Spore trap

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

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收录情况： SCI

摘要： In this study, a real-time PCR assay was established to quantify airborne inoculum of Blumeria graminis f. sp. tritici (Bgt), the causal agent of wheat powdery mildew. Burkard 7-day recording spore traps were used to collect Bgt spores in air in Langfang City, Hebei Province, China. A linear relationship between number of spores counted with a compound microscope and those determined with the real-time PCR assay was obtained in artificially inoculated spore trap tape sections. The dynamics of airborne conidia concentrations of Bgt were similar. There were significant correlations (P < 0.01) between spore concentrations by using a compound microscope and the real-time PCR assay in three growing seasons. The results demonstrated a potential method to quantitatively determine spore inoculum potential in wheat fields by using a real-time PCR assay.

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