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The accumulation and localization of chalcone synthase in grapevine (Vitis vinifera L.)

文献类型： 外文期刊

作者： Wang, Huiling 1 ; Wang, Wei 3 ; Zhan, JiCheng 2 ; Yan, Ailing 4 ; Sun, Lei 1 ; Zhang, Guojun 1 ; Wang, Xiaoyue 1 ; Ren, J 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 100097, Peoples R China

2.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

3.Chinese Acad Forestry, Inst Forestry, State Forestry Adm, Key Lab Silviculture, Beijing 100091, Peoples R China

4.Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing, Peoples R China

关键词： Flavonoids;Chalcone synthase;Immunolocalization;Grapevine

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Chalcone synthase (CHS, E.C.2.3.1.74) is the first committed enzyme in the flavonoid pathway. Previous studies have primarily focused on the cloning, expression and regulation of the gene at the transcriptional level. Little is yet known about the enzyme accumulation, regulation at protein level, as well as its localization in grapevine. In present study, the accumulation, tissue and subcellular localization of CHS in different grapevine tissues (Vitis vinifera L. Cabernet Sauvignon) were investigated via the techniques of Western blotting, immunohistochemical localization, immunoelectron microscopy and confocal microscopy. The results showed that CHS were mainly accumulated in the grape berry skin, leaves, stem tips and stem phloem, correlated with flavonoids accumulation. The accumulation of CHS is developmental dependent in grape berry skin and flesh. Immunohistochemical analysis revealed that CHS were primarily localized in the exocarp and vascular bundles of the fruits during berry development; in palisade, spongy tissues and vascular bundles of the leaves; in the primary phloem and pith ray in the stems; in the growth point, leaf primordium, and young leaves of leaf buds; and in the endoderm and primary phloem of grapevine roots. Furthermore, at the subcellular level, the cell wall, cytoplasm and nucleus localized patterns of CHS were observed in the grapevine vegetative tissue cells. Results above indicated that distribution of CHS in grapevine was organ-specific and tissue-specific. This work will provide new insight for the biosynthesis and regulation of diverse flavonoid compounds in grapevine. (C) 2016 Elsevier Masson SAS. All rights reserved.

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