您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

High-level expression of a novel chromoplast phosphate transporter ClPHT4;2 is required for flesh color development in watermelon

文献类型： 外文期刊

作者： Zhang, Jie 1 ; Guo, Shaogui 1 ; Ren, Yi 1 ; Zhang, Haiying 1 ; Gong, Guoyi 1 ; Zhou, Ming 2 ; Wang, Guizhang; Zong, Me 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Key Lab Biol & Genet Improvement Hort Crops North, Natl Engn Res Ctr Vegetables, Beijing Key Lab Vegetable Germplasm Improvement, Beijing 100097, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Key Lab Bio

关键词： chromoplast;Citrullus lanatus;flesh color;phosphate transporter;transcriptional regulation

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.151； 五年影响因子：10.475 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Chromoplast development plays a crucial role in controlling carotenoid content in watermelon flesh. Modern cultivated watermelons with colorful flesh are believed to originate from pale-colored and no-sweet progenitors. But the molecular basis of flesh color formation and regulation is poorly understood. More chromoplasts and released carotenoid globules were observed in the red-fleshed fruit of the 97103 cultivar than in the pale-colored fruits of the PI296341-FR line. Transcriptome profiles of these two materials identified Cla017962, predicted as ClPHT4;2, was dramatically up-regulated during flesh color formation. High ClPHT4;2 expression levels were closely correlated with increased flesh carotenoid contents among 198 representative watermelon accessions. Down-regulation of ClPHT4;2 expression in transgenic watermelons reduced the fruit carotenoid accumulation. ClPHT4;2 as a function of chromoplast-localized phosophate transporter was tested by heterologous expression into a yeast phosphate-uptake-defective mutant, western blotting, subcellular localization, and immunogold electron microscopy analysis. Two transcription factors, ClbZIP1 and ClbZIP2, were identified, which responded to ABA and sugar signaling to regulate ClPHT4;2 transcription only in cultivated watermelon species. Our findings suggest that elevated ClPHT4;2 gene expression is necessary for carotenoid accumulation, and may help to characterize the co-development of flesh color and sweetness during watermelon development and domestication.

  • 相关文献

[1]Non-destructive assessment of quality parameters in 'Friar' plums during low temperature storage using visible/near infrared spectroscopy. Li, Ming,Lv, Wenbo,Zhao, Rui,Guo, Huixin,Han, Donghai,Liu, Jing.

[2]Mutation in the gene encoding 1-aminocyclopropane-1-carboxylate synthase 4 (CitACS4) led to andromonoecy in watermelon. Ji, Gaojie,Zhang, Jie,Zhang, Haiying,Sun, Honghe,Gong, Guoyi,Shi, Jianting,Tian, Shouwei,Guo, Shaogui,Ren, Yi,Xu, Yong,Ji, Gaojie,Shen, Huolin,Gao, Junping. 2016

[3]Dynamic characteristics of enzymes and transcriptome related to sugar metabolism and accumulation in sweet and non-sweet watermelon fruits. Xu, Y.,Guo, S.,Liu, J.,He, H.,Zhang, H.,Ren, Y.,Sun, H.,Gong, G.,Fei, Z.,Zheng, Y.,Huang, M.,Zhong, S.,Liu, J.. 2012

[4]Identification and validation of a core set of microsatellite markers for genetic diversity analysis in watermelon, Citrullus lanatus Thunb. Matsum. & Nakai. Zhang, Haiying,Wang, Hui,Guo, Shaogui,Ren, Yi,Gong, Guoyi,Xu, Yong,Weng, Yiqun.

[5]Comparative transcriptome profiling of potassium starvation responsiveness in two contrasting watermelon genotypes. Fan, Molin,Huang, Yuan,Zhong, Yaqin,Kong, Qiusheng,Xie, Junjun,Niu, Mengliang,Bie, Zhilong,Xu, Yong.

[6]Dynamic characteristics of sugar accumulation and related enzyme activities in sweet and non-sweet watermelon fruits. Liu, Jingan,Guo, Shaogui,He, Hongju,Zhang, Haiying,Gong, Guoyi,Ren, Yi,Xu, Yong,Liu, Jingan,Guo, Shaogui,He, Hongju,Zhang, Haiying,Gong, Guoyi,Ren, Yi,Xu, Yong.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台