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High-level expression of a novel chromoplast phosphate transporter ClPHT4;2 is required for flesh color development in watermelon

文献类型： 外文期刊

作者： Zhang, Jie 1 ; Guo, Shaogui 1 ; Ren, Yi 1 ; Zhang, Haiying 1 ; Gong, Guoyi 1 ; Zhou, Ming 2 ; Wang, Guizhang; Zong, Me 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Key Lab Biol & Genet Improvement Hort Crops North, Natl Engn Res Ctr Vegetables, Beijing Key Lab Vegetable Germplasm Improvement, Beijing 100097, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Key Lab Bio

关键词： chromoplast;Citrullus lanatus;flesh color;phosphate transporter;transcriptional regulation

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.151； 五年影响因子：10.475 ）

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收录情况： SCI

摘要： Chromoplast development plays a crucial role in controlling carotenoid content in watermelon flesh. Modern cultivated watermelons with colorful flesh are believed to originate from pale-colored and no-sweet progenitors. But the molecular basis of flesh color formation and regulation is poorly understood. More chromoplasts and released carotenoid globules were observed in the red-fleshed fruit of the 97103 cultivar than in the pale-colored fruits of the PI296341-FR line. Transcriptome profiles of these two materials identified Cla017962, predicted as ClPHT4;2, was dramatically up-regulated during flesh color formation. High ClPHT4;2 expression levels were closely correlated with increased flesh carotenoid contents among 198 representative watermelon accessions. Down-regulation of ClPHT4;2 expression in transgenic watermelons reduced the fruit carotenoid accumulation. ClPHT4;2 as a function of chromoplast-localized phosophate transporter was tested by heterologous expression into a yeast phosphate-uptake-defective mutant, western blotting, subcellular localization, and immunogold electron microscopy analysis. Two transcription factors, ClbZIP1 and ClbZIP2, were identified, which responded to ABA and sugar signaling to regulate ClPHT4;2 transcription only in cultivated watermelon species. Our findings suggest that elevated ClPHT4;2 gene expression is necessary for carotenoid accumulation, and may help to characterize the co-development of flesh color and sweetness during watermelon development and domestication.

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