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Molecular cloning and expression analysis of the sucrose transporter gene family from Theobroma cacao L.

文献类型： 外文期刊

作者： Li, Fupeng 1 ; Wu, Baoduo 1 ; Qin, Xiaowei 1 ; Yan, Lin 1 ; Hao, Chaoyun 1 ; Tan, Lehe 1 ; Lai, Jianxiong 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Wanning 571533, Peoples R China; Minist Agr, Key Lab Genet Resources Utilizat Spice & Beverage, Wanning 571533, Peoples R China; Hainan Prov Key Lab Genet Improvement & Qual Regu, Wanning 571533, Peoples R China

关键词： Sucrose transporter;Theobroma cacao;Expression pattern;Cloning

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

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收录情况： SCI

摘要： In this study, we performed cloning and expression analysis of six putative sucrose transporter genes, designated TcSUT1, TcSUT2, TcSUT3, TcSUT4, TcSUT5 and TcSUT6, from the cacao genotype 'TAS-R8'. The combination of cDNA and genomic DNA sequences revealed that the cacao SLIT genes contained exon numbers ranging from 1 to 14. The average molecular mass of all six deduced proteins was approximately 56 kDa (range 52 to 66 kDa). All six proteins were predicted to exhibit typical features of sucrose transporters with 12 trans-membrane spanning domains. Phylogenetic analysis revealed that TcSUT2 and TcSUT4 belonged to Group 2 SLIT and Group 4 SUT, respectively, and the other TcSUT proteins were belonging to Group 1 SLIT. Real-time PCR was conducted to investigate the expression pattern of each member of the SUT family in cacao. Our experiment showed that TcSUT1 was expressed dominantly in pods and that, TcSUT3 and TcSUT4 were highly expressed in both pods and in bark with phloem Within pods, TcSUT1 and TcSUT4 were expressed more in the seed coat and seed from the pod enlargement stage to the ripening stage. TcSUT5 expression sharply increased to its highest expression level in the seed coat during the ripening stage. Expression pattern analysis indicated that TcSUT genes may be associated with photoassimilate transport into developing seeds and may, therefore, have an impact on seed production. (C) 2014 Elsevier B.V. All rights reserved.

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