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Optimization of Enzyme-assisted Extraction of Antitumor Polysaccharides from Hericium erinaceus mycelia

文献类型： 外文期刊

作者： Qin, Yi 1 ; Zhang, Zuofa 2 ; Song, Tingting 2 ; Lv, Guoying 2 ;

作者机构： 1.Zhejiang Univ, Ind Technol Res Inst, Technol Transfer Ctr, Hangzhou 310058, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Hericium erinaceus mycelia;enzyme-assisted extraction;polysaccharides;antitumor activity

期刊名称：FOOD SCIENCE AND TECHNOLOGY RESEARCH （ 影响因子：0.668； 五年影响因子：0.895 ）

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收录情况： SCI

摘要： In this study, an efficient complex enzyme-assisted extraction technology was developed and optimized to extract polysaccharides from Hericium erinaceus mycelia (HEP) using the Box-Behnken design based on the results of single factor experiments. As a result, the optimal protocol was as follows: an extraction time of 79 min, an extraction temperature of 50 degrees C, a pH of 5.7 and a ratio of water to raw materials of 33.4 mL/g. These optimal extraction conditions achieved the highest yield (13.9 +/- 0.3%) of HEP. The purified fraction (HEP-2) was composed of glucose, galactose, mannose, fucose, arabinose, xylose and rhammnose. HEP-2 manifested an obvious inhibitory effect on the growth of HeLa cells. The overall findings implied that enzyme-assisted procedure is a superior method and that HEP-2 might be useful for developing natural and safe antitumor drugs.

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