Study on external factors affecting egg yolk plasma by asymmetrical flow field-flow fractionation
文献类型： 外文期刊
作者： Dou, Haiyang 1 ; Zhang, Xiaoyue 2 ; Zhang, Aixia 3 ; Choi, Jaeyeong 4 ; Wang, Jing 1 ; Shen, Shigang 1 ; Zhang, Wenhui 2 ;
作者机构： 1.Hebei Univ, Coll Chem & Environm Sci, Key Lab Analyt Sci & Technol Hebei Prov, Baoding 071002, Peoples R China
2.Hebei Univ, Sch Med, Baoding 071002, Peoples R China
3.Hebei Acad Agr & Forestry Sci, Inst Millet Crops, Natl Millet Improvement Ctr, Key Lab Minor Cereal Crops Hebei Prov, Shijiazhuang 050035, Peoples R China
4.Hannam Univ, Dept Chem, Daejeon 34054, South Korea
关键词： Asymmetrical flow field-flow fractionation;Light scattering;Egg yolk plasma;Low density lipoprotein;Aggregation
期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：6.475； 五年影响因子：6.508 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： In this work, the ability of asymmetrical flow field-flow fractionation (AF4) coupled with UV-vis, multiangle light scattering (MALS), and quasi-elastic light scattering (QELS) (AF4-UV-MALS-QELS) for monitoring aggregation of components of egg yolk plasma was evaluated. The effect of external factors (Le., pH, storage conditions, freezing and heat treatments) on the egg yolk plasma was studied. The results reveal that the aggregation mechanism of components of egg yolk plasma during heat and freezing treatment is different. The results suggest that the low density lipoproteins (LDLs) in egg yolk plasma undergo a 'clusters-fusion-gel' process under heat treatment at pH 10. The alkaline conditions promote the formation of LDL aggregates. Also, storage conditions play a role in the formation of LDL aggregates. It was found that the hen eggs stored for 7 days at room temperature contain less aggregate than those stored at 4 degrees C for the same period. The combination of AF4 with online MALS-QELS provided conformational information in terms of the shape and size distribution of LDL aggregates. AF4-UV-MALS-QELS was proved to be a rapid and gentle method for the separation and characterization of egg yolk plasma aggregates. (C) 2017 Elsevier Ltd. All rights reserved.
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