Functional characterization of a pheromone-binding protein from rice leaffolder Cnaphalocrocis medinalis in detecting pheromones and host plant volatiles
文献类型： 外文期刊
作者： Sun, X. 1 ; Zhao, Z. -F. 1 ; Zeng, F. -F. 1 ; Zhang, A. 3 ; Lu, Z. -X. 4 ; Wang, M. -Q. 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan, Peoples R China
2.Henan Univ, Coll Life Sci, Key Lab Invas Ecol, Kaifeng, Henan, Peoples R China
3.USDA ARS, BARC West, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA
4.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou, Zhejiang, Peoples R China
关键词： Cnaphalocrocis medinalis;pheromone-binding proteins;immunocytochemistry;fluorescence competitive binding assays;trichoid sensilla
期刊名称：BULLETIN OF ENTOMOLOGICAL RESEARCH （ 影响因子：1.75； 五年影响因子：2.007 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Pheromone-binding proteins (PBPs) are believed to be involved in the recognition of semiochemicals. In the present study, western blot analysis, fluorescence-binding characteristics and immunolocalization of CmedPBP4 from the rice leaffolder, Cnaphalocrocis medinalis, were investigated. Western blot analysis revealed that CmedPBP4 showed obvious antenna-specific expression patterns in female and male antenna, and made a clearly different sex-biased expression. Immunocytochemical labeling revealed that CmedPBP4 showed specific expression in the trichoid sensilla. Competitive fluorescence binding assays indicated that CmedPBP4 could selectively recognize three sex pheromone components (Z13-18: Ac, Z11-16: Al and Z13-18: OH) and eleven rice plant volatiles, including cyclohexanol, nerolidol, cedrol, dodecanal, ionone, (-)-alpha-cedrene, (Z)-farnesene, beta-myrcene, R-(+)-limonene, (-)-limonene, and (+)-3-carene. Meanwhile the CmedPBP4 detection of sex pheromones and host odorants was pH-dependent. Our results, for the first time, provide further evidence that trichoid sensilla might be play an important role in detecting sex pheromones and host plant volatiles in the C. medinalis moth. Our systematic studies provided further detailed evidence for the function of trichoid sensilla in insect semiochemical perception.
- 相关文献
作者其他论文 更多>>
-
Three chemosensory proteins from the rice leaf folder Cnaphalocrocis medinalis involved in host volatile and sex pheromone reception
作者：Zeng, F. -F.;Liu, H.;Abdelnabby, H.;Wang, M. -Q.;Zhang, A.;Lu, Z. -X.;Leal, W. S.;Abdelnabby, H.
关键词：chemosensory proteins; binding properties; immunolocation labelling; RNA interference; EAG response
-
Interactions of two odorant-binding proteins influence insect chemoreception
作者：Sun, X.;Zeng, F. -F.;Yan, M. -J.;Wang, M. -Q.;Sun, X.;Zhang, A.;Lu, Z. -X.
关键词：odorant-binding protein;Cnaphalocrocis medinalis;molecular docking;fluorescence competitive binding assays;RNA interference;interactions;electroantennogram responses
