Embryo induction and plant regeneration of Callerya speciosa (Fabaceae) through anther culture
文献类型： 外文期刊
作者： Huang, Bilan 1 ; Xu, Li 1 ; Li, Kelie 1 ; Fu, Yunlu 1 ; Li, Zhiying 2 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Key Lab Trop Crops Germplasm Resources Genet Impr, Key Lab Crop Gene Resources & Germplasm Enhanceme, Inst Trop Crops Genet Resources,Minist Agr, Danzhou 571737, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Key Lab Trop Crops Germplasm Resources Genet Impr, Key Lab Crop Gene Resources & Germplasm Enhanceme, Inst Trop Crops Genet Resources,Minist
关键词： anther;C. speciosa (Fabaceae);embryogenesis;plant regeneration
期刊名称：AUSTRALIAN JOURNAL OF BOTANY （ 影响因子：1.237； 五年影响因子：1.394 ）
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收录情况： SCI
摘要： An in vitro protocol for Callerya speciosa (Champ.) Schot regeneration through embryogenesis was developed using the anthers as the explants. The late uninucleate stage of the microspore was optimal for the anther culture of C. speciosa. Embryonic callus was induced on a MS basal medium supplemented with 4.4 mu M 6-benzylaminopurine (BA) and 9.04 mu M 2,4-dichlorophenoxyacetic acid (2,4-D). Embryos were obtained on MS medium supplemented with 2.2 mu M BA and 0.5 mu M naphthaleneacetic acid (NAA). The highest percentage (16.7%) of embryos was achieved using the culture medium MS + 0.25 mu M NAA + 1.1 mu M BA. The highest percentage of embryos that developed into plants was 18.3%. However, haploid plants were not observed, which may have been due to the collection of the calli from the anther wall. The results presented here demonstrate the establishment of a highly efficient and rapid system for regenerating C. speciosa using anther cultures.
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