Novel microsatellite markers for the oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae) and effects of null alleles on population genetics analyses
文献类型： 外文期刊
作者： Song, W. 1 ; Cao, L. -J. 1 ; Wang, Y. -Z. 1 ; Li, B. -Y. 1 ; Wei, S. -J. 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China
2.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 071000, Peoples R China
3.Southwest Forestry Univ, Coll Forestry, Key Lab Forest Disaster Warning & Control Yunnan, Kunming 650224, Peoples R China
关键词： Grapholita molesta;next-generation sequencing;SSR;null allele;population genetic structure
期刊名称：BULLETIN OF ENTOMOLOGICAL RESEARCH （ 影响因子：1.75； 五年影响因子：2.007 ）
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收录情况： SCI
摘要： The oriental fruit moth (OFM) Grapholita molesta (Lepidoptera: Tortricidae) is an important economic pest of stone and pome fruits worldwide. We sequenced the OFM genome using next-generation sequencing and characterized the microsatellite distribution. In total, 56,674 microsatellites were identified, with 11,584 loci suitable for primer design. Twenty-seven polymorphic microsatellites, including 24 loci with trinucleotide repeat and three with pentanucleotide repeat, were validated in 95 individuals from four natural populations. The allele numbers ranged from 4 to 40, with an average value of 13.7 per locus. A high frequency of null alleles was observed in most loci developed for the OFM. Three marker panels, all of the loci, nine loci with the lowest null allele frequencies, and nine loci with the highest null allele frequencies, were established for population genetics analyses. The null allele influenced estimations of genetic diversity parameters but not the OFM's genetic structure. Both a STRUCTURE analysis and a discriminant analysis of principal components, using the three marker panels, divided the four natural populations into three groups. However, more individuals were incorrectly assigned by the STRUCTURE analysis when the marker panel with the highest null allele frequency was used compared with the other two panels. Our study provides empirical research on the effects of null alleles on population genetics analyses. The microsatellites developed will be valuable markers for genetic studies of the OFM.
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