您好,欢迎访问中国热带农业科学院 机构知识库!

Molecular cloning and characterization of S-adenosylmethionine decarboxylase gene in rubber tree (Hevea brasiliensis)

文献类型: 外文期刊

作者: Zhao, Manman 1 ; Liu, Hui 1 ; Deng, Zhi 1 ; Chen, Jiangshu 1 ; Yang, Hong 1 ; Li, Huiping 3 ; Xia, Zhihui 4 ; Li, Dejun 1 ;

作者机构: 1.Chinese Acad Trop Agr Sci, Key Lab Biol & Genet Resources Rubber Tree, Minist Agr, Rubber Res Inst, Baodao Xincun 571737, Danzhou, Peoples R China

2.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan 430070, Peoples R China

3.Hainan Univ, Coll Agr, Haikou 570228, Peoples R China

4.Haina

关键词: Rubber tree;Polyamines;S-Adenosylmethionine decarboxylase;Translational regulation;Genomic structure

期刊名称:PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS ( 影响因子:2.391; 五年影响因子:2.836 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: 5-Adenosylmethionine decarboxylase (SAMDC) is a key rate-limiting enzyme involved in polyamines biosynthesis, and it plays important roles in plant growth, development and stresses response. However, no SAMDC gene was reported in rubber tree. Here wereport characteristics of an SAMDC gene (HbSAMDCl) in rubber tree. HbSAMDCl contains a 1080 bp open reading frame (ORF) encoding 359 amino acids. Quantitative real-time PCR analyses revealed that HbSAMDCl exhibited distinct expression patterns in different tissues and was regulated by various stresses, including drought, cold, salt, wounding, and H_20_2 treatments. HbSAMDCl 5' untranslated region (UTR) contains a highly conserved overlapping tiny and small upstream ORFs (uORFs), encoding 2 and 52 aminoacid residues, respectively. No introns were located in the main ORF of HbSAMDCl, whereas two introns were found in the 5' UTR. In transgenic tobaccos, the highly conserved small uORF of HbSAMDCl is found to be responsible for translational repression ofdownstream (3-glucuronidase reporter. To our knowledge, this is the first report on molecular cloning, expression profiles, and 5' UTR characteristics of HbSAMDCl. These results lay solidfoundation for further elucidating HbSAMDCl function in rubber tree.

  • 相关文献

[1]橡胶树炭疽病菌Cap2移基因的克隆和序列分析. 林春花,李超萍,李博勋,周维,黄贵修. 2012

[2]Differential Responses of Polyamines and Antioxidants to Drought in a Centipedegrass Mutant in Comparison to Its Wild Type Plants. Liu, Mingxi,Liu, Mingxi,Chen, Jingjing,Lu, Shaoyun,Chen, Jingjing,Guo, Zhenfei. 2017

[3]De novo assembly and characterization of bark transcriptome using Illumina sequencing and development of EST-SSR markers in rubber tree (Hevea brasiliensis Muell. Arg.). Li, Dejun,Deng, Zhi,Qin, Bi,Liu, Xianghong,Men, Zhonghua. 2012

[4]Design of natural rubber precision ditch fertilization machine. Wang, Yeqin,Deng, Yiguo,Zhang, Yuan,Wei, Lijiao. 2017

[5]Gain-of-function in Arabidopsis (GAINA) for identifying functional genes in Hevea brasiliensis. Cheng, Han,Gao, Jing,Cai, Haibin,Zhu, Jianshun,Huang, Huasun,Cheng, Han,Huang, Huasun. 2016

[6]Molecular mechanism of ethylene stimulation of latex yield in rubber tree (Hevea brasiliensis) revealed by de novo sequencing and transcriptome analysis. Liu, Jin-Ping,Zhuang, Yu-Fen,Guo, Xiu-Li,Li, Yi-Jian. 2016

[7]Identification of novel microRNAs in Hevea brasiliensis and computational prediction of their targets. Gebelin, Virginie,Argout, Xavier,Engchuan, Worrawat,Pitollat, Bertrand,Duan, Cuifang,Montoro, Pascal,Leclercq, Julie,Engchuan, Worrawat,Duan, Cuifang. 2012

[8]Transcriptome sequencing and analysis of rubber tree (Hevea brasiliensis Muell.) to discover putative genes associated with tapping panel dryness (TPD). Liu, Jin-Ping,Tian, Xiao-Yan,Xia, Zhi-Qiang,Li, Yi-Jian. 2015

[9]Characterization of HbEREBP1, a wound-responsive transcription factor gene in laticifers of Hevea brasiliensis Muell. Arg.. Chen, Yue-Yi,Wang, Li-Feng,Dai, Long-Jun,Yang, Shu-Guang,Tian, Wei-Min,Chen, Yue-Yi.

[10]The small RNA profile in latex from Hevea brasiliensis trees is affected by tapping panel dryness. Gebelin, Virginie,Leclercq, Julie,Argout, Xavier,Sarah, Gautier,Montoro, Pascal,Kuswanhadi,Chaidamsari, Tetty,Hu, Songnian,Yang, Meng,Tang, Chaorong. 2013

[11]Ultrasound-assisted tapping of latex from Para rubber tree Hevea brasiliensis. She, Fenghua,Wang, Jin,An, Feng,Lin, Weifu,Zhu, Deming,She, Fenghua,Kong, Lingxue,An, Feng. 2013

[12]Characterization of Sugar Contents and Sucrose Metabolizing Enzymes in Developing Leaves of Hevea brasiliensis. Zhu, Jinheng,Qi, Jiyan,Fang, Yongjun,Xiao, Xiaohu,Lan, Jixian,Tang, Chaorong,Zhu, Jinheng,Lan, Jixian,Tang, Chaorong,Li, Jiuhui. 2018

[13]Next-generation sequencing, assembly, and comparative analyses of the latex transcriptomes from two elite Hevea brasiliensis varieties. Li, Dejun,Liu, Hui,Zhao, Manman,Deng, Zhi,Li, Yu,Zeng, Rizhong,Tian, Weimin,Hao, Lili,Zhao, Manman. 2015

[14]Proteome analysis of interaction between rootstocks and scions in Hevea brasiliensis. Yuan, Kun,Yang, Li-Fu,Wang, Zhen-Hui,Lin, Wei-Fu,Cao, Jian-Hua,Yuan, Kun,Yang, Li-Fu,Wang, Zhen-Hui,Lin, Wei-Fu,Cao, Jian-Hua,Ding, Xuan. 2011

[15]Regulation of MIR Genes in Response to Abiotic Stress in Hevea brasiliensis. Gebelin, Virginie,Leclercq, Julie,Montoro, Pascal,Hu, Songnian,Tang, Chaorong. 2013

[16]Identification, Functional Study, and Promoter Analysis of HbMFT1, a Homolog of MFT from Rubber Tree (Hevea brasiliensis). Bi, Zhenghong,Bi, Zhenghong,Huang, Huasun,Hua, Yuwei,Li, Xiang. 2016

[17]Development, characterization, genetic diversity and cross-species/genera transferability of ILP markers in rubber tree (Hevea brasiliensis). Li, Dejun,Deng, Zhi,Xia, Zhihui,Liu, Xianghong,Feng, Fuying.

[18]Identification of genes differentially expressed in the roots of rubber tree (Hevea brasiliensis Muell. Arg.) in response to phosphorus deficiency. He, Peng,Qin, Huaide,Wu, Min,Wu, Bingsun,Wei, Jiashao,Wang, Dapeng.

[19]Development and characterization of intron-flanking EST-PCR markers in rubber tree (Hevea brasiliensis Muell. Arg.). Li, Dejun,Deng, Zhi,Liu, Xianghong,Xia, Zhihui,Dong, Junmei,Feng, Fuying.

[20]Cloning and characterization of HbMT2a, a metallothionein gene from Hevea brasiliensis Muell. Arg differently responds to abiotic stress and heavy metals. Li, Yan,Chen, Yue Yi,Yang, Shu Guang,Tian, Wei Min.

作者其他论文 更多>>