Identification of epitopes on nonstructural protein 7 of porcine reproductive and respiratory syndrome virus recognized by monoclonal antibodies using phage-display technology
文献类型： 外文期刊
作者： Wang, Heng 1 ; Liu, Rongchang 4 ; Zhang, Weidong 5 ; Sun, Lingshuang 1 ; Ning, Zhangyong 1 ; Ji, Fangxiao 3 ; Cui, Jin 3 ;
作者机构： 1.South China Agr Univ, Coll Vet Med, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China
2.Guangdong Prov Key Lab Prevent & Control Severe C, Guangzhou 510642, Guangdong, Peoples R China
3.MOA Key Lab Anim Vaccine Dev, Guangzhou 510642, Guangdong, Peoples R China
4.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian Province, Peoples R China
5.Hosp South China Agr
关键词： Porcine reproductive and respiratory syndrome virus;Nonstructural protein 7;Epitopes;Phage-display technology;Antigenic structure
期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）
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收录情况： SCI
摘要： Nonstructural protein 7 (nsp7) of porcine reproductive and respiratory syndrome virus (PRRSV) is considered to be a suitable reagent for the development of serological diagnostic assays. It can be expressed as a soluble recombinant protein in Escherichia coli, and its antibody response may continue up to 202 days post-infection. Furthermore, the region encoded by nsp7 is highly homologous among various strains within the genotype, and the results of nsp7-based enzyme-linked immunosorbent assay (ELISA) showed high agreement with previous Idexx ELISA results. All these evidences suggest the existence of important epitopes on nsp7, though the characteristics of these epitopes remain unclear. In the present study, we prepared three monoclonal antibodies against nsp7 protein and used them to screen the epitope-distribution characteristics of PRRSV nsp7 protein by phage-display technology. We identified a linear epitope NAWGDEDRLN at amino acids 153-162 type II PRRSV nsp7 beta subunit. This newly defined epitope showed excellent reactivity with PRSSV-positive serum samples. These results further our understanding of the antigenic structure of nsp7 protein, and provide efficient reagents for PRRSV serological tests.
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