文献类型： 外文期刊

作者： Jiang, Ming ¹ ; Jiang, Jing-Jing ² ; Miao, Li-Xiang ³ ; He, Cai-Ming ¹ ;

作者机构： 1.Taizhou Univ, Coll Life Sci, Key Discipline Zhejiang Prov, Jiaojiang 318000, Peoples R China

2.Chinese Univ Hong Kong, State Key Lab Agrobiotechnol Shenzhen Base, Shenzhen Res Inst, Shenzhen 518057, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Brassica oleracea var. italica;C3H-type zinc finger;Salt stress;BoC3H;Over-expression

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

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收录情况： SCI

摘要： C3H-type zinc finger proteins play important roles in plant growth, development, and stress responses. A C3H-type zinc finger gene, designated BoC3H, was isolated from broccoli (Brassica oleracea var. italica). The complete coding sequence of BoC3H was 1074 bp in length, encoding 357 amino acids with two CCCH motifs of C-X-7-C-X-5-C-X-3-H and C-X-5-C-X-4-C-X-3-H. The transcripts of BoC3H were profoundly induced by NaCl, and the highest expression level was observed at 18 h after treatment. Four broccoli lines over-expressing the BoC3H gene were obtained, and they exhibited higher germination rates, dry weight and chlorophyll content in response to salt stress as compared to those of wild type plants. Over-expression of BoC3H significantly decreased hydrogen peroxide (H2O2) level, relative electrical conductivity (REC) and malondialdehyde (MDA) contents, but dramatically increased free proline content, catalase, peroxidase and superoxide dismutase enzyme activities, resulting in less cell death in the leaves of transgenic plants. Taken together, our results suggest that BoC3H is likely to contribute to salt stress tolerance by regulating H2O2, REC, free proline, MDA and antioxidant enzyme levels in broccoli.