Application and comparison in biosynthesis and biodegradation by Fusarium solani and Aspergillus fumigatus cutinases
文献类型: 外文期刊
作者: Ping, Li-Feng 1 ; Chen, Xiao-Yang 2 ; Yuan, Xiao-Li 1 ; Zhang, Min 1 ; Chai, Yan-Jun 1 ; Shan, Sheng-Dao 1 ;
作者机构: 1.Zhejiang Univ Sci & Technol, Key Lab Recycling & Ecotreatment Waste Biomass Zh, Hangzhou 310023, Zhejiang, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Environm Resource & Soil Fertilizer, Hangzhou 310021, Zhejiang, Peoples R China
关键词: F. solani;A. fumigatus;Cutinase;Characterization;Biodegradation;Biosynthesis
期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:6.953; 五年影响因子:6.737 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: In this study, two synthesized cutinase genes from Fusarium solani and Aspergillus fumigatus were expressed in Pichia pastoris X33. The characteristics of these two cutinases were investigated and compared. The results indicated that F. solani and A. fumigatus cutinases hydrolyzed p-nitrophenyl substrates with different carbon chain lengths. A. fumigatus cutinase predominately hydrolyzed p-nitrophenyl butyrate, but F. solani cutinase preferred p-nitrophenyl decanoate. The abilities of polymer synthesis and bioplastic degradation were tested and compared between F. solani and A. fumigatus cutinases. The results showed that F. solani cutinase had degradation ability on poly(epsilon-caprolactone) (PCL) and synthesized polymer with a molecular weight (MW) of 2300 in organic solvent. However, A. fumigatus cutinase completely degraded PCL and synthesized molecules with a MW of 25,000, suggesting that A. fumigatus cutinase has more promising applications. (C) 2017 Elsevier B.V. All rights reserved.
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