Generation of Newly Discovered Resistance Gene mcr-1 Knockout in Escherichia coli Using the CRISPR/Cas9 System
文献类型： 外文期刊
作者： Sun, Lichang 1 ; He, Tao 1 ; Zhang, Lili 1 ; Pang, Maoda 1 ; Zhang, Qiaoyan; Zhou, Yan 2 ; Bao, Hongduo; Wang, Ran;
作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Control Technol & Standard Agroprod Safet, Minist Agr,Key Lab Food Qual & Safety Jiangsu Pro, State Key Lab Breeding Base,Inst Food Qual & Safe, Nanjing 210014, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Key Lab Control Technol & Standard Agroprod Safet, Minist Agr,Key Lab Food Qual & Safety Jiangsu Pro, State Key Lab Breeding Base,Inst
关键词： mcr-1;knockout;E. coli;CRISPR/Cas9 system;BMAP-27
期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）
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收录情况： SCI
摘要： The mcr-1 gene is a new "superbug" gene discoverd in China in 2016 that makes bacteria highly resistant to the last-resort class of antibiotics. The mcr-1 gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy using the CRISPR/Cas9-mediated approach that can efficiently induce mcr-1 gene knockout in Escherichia coli. Our findings suggested that using the CRISPR/Cas9 system to knock out the resistance gene mcr-1 might be a potential anti-resistant strategy. Bovine myeloid antimicrobial peptide-27 could help deliver plasmid pCas:: mcr targeting specific DNA sequences of the mcr-1 gene into microbial populations.
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