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Identification and sex expression profiling of odorant-binding protein genes in Trichogramma japonicum (Hymenoptera: Trichogrammatidae) using RNA-Seq

文献类型： 外文期刊

作者： Wu, Jia-Dong 1 ; Shen, Zhao-Can 1 ; Hua, Hai-Qing 1 ; Zhang, Fan 2 ; Li, Yuan-Xi 1 ;

作者机构： 1.Nanjing Agr Univ, Dept Entomol, Nanjing 210095, Jiangsu, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

3.Nanjing Agr Univ, Coll Plant Protect, 1 Weigang, Nanjing 210095, Jiangsu, Peoples R China

关键词： Trichogramma japonicum;Transcriptome;Odorant binding proteins;qRT-PCR;Sex

期刊名称：APPLIED ENTOMOLOGY AND ZOOLOGY （ 影响因子：1.403； 五年影响因子：1.381 ）

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收录情况： SCI

摘要： Trichogramma japonicum Ashmead (Hymenoptera: Trichogrammatidae) is a biological control agent that parasitizes the eggs of many lepidopteran insects. The control efficiency is closely related to the ability of the parasitoid to use chemical volatiles in searching for hosts, and the odorant binding proteins (OBPs) may serve as a first step in the perception of these chemical cues. Understanding the ability of Trichogramma to detect relevant host odorant cues might help to design better strategies to control target lepidopteran insect pests. In the present study, we assembled the transcriptome of T. japonicum using Illumina sequencing technology and identified 15 putative OBP genes. All the OBP genes have complete open reading frames and contain six conserved cysteines. In addition, sex-biased expression was found in eight OBP genes by using quantitative real-time PCR, which suggested different functions of these genes. Phylogenetic analyses revealed that Hymenoptera OBP genes were divided into Classic, Minus-C, and Double Minus-C subfamilies. All the identified OBP genes of T. japonicum belong to the Classic subfamily. These results provide an important foundation for a better understanding of the complex chemoreception system of T. japonicum.

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