Reversed-Phase High-Performance Liquid Chromatography for the Quantification and Optimization for Extracting 10 Kinds of Carotenoids in Pepper (Capsicum annuum L.) Leaves
文献类型： 外文期刊
作者： Li, Jing 1 ; Xie, Jianming 1 ; Yu, Jihua 1 ; Lv, Jian 1 ; Zhang, Junfeng 1 ; Wang, Xiaolong 1 ; Wang, Cheng 1 ; Tang, Cha 1 ;
作者机构： 1.Gansu Agr Univ, Coll Hort, Lanzhou 730070, Gansu, Peoples R China
2.Gansu Acad Agr Sci, Vegetable Inst, Lanzhou 730070, Gansu, Peoples R China
3.Univ Dev Studies, Dept Hort, Fac Agr FoA, POB TL 1882, Tamale, Ghana
关键词： pepper leaves;carotenoid;high performance liquid chromatography;separation and quantification;optimization;ultrasound-assisted extraction
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： Carotenoids are considered to be crucial elements in many fields and, furthermore, the significant factor in pepper leaves under low light and chilling temperature. However, little literature focused on the method to determinate and extract the contents of carotenoid-compositions in pepper leaves. Therefore, a time-saving and highly sensitive reversed-phase high-performance liquid chromatography method for separation and quantification of 10 carotenoids was developed, and an optimized technological process for carotenoid composition extraction in pepper leaves was established for the first time. Our final method concluded that six xanthophylls eluted after about 9-26 min. In,contrast, four carotenes showed higher retention times after nearly 28-40 min, which significantly shortened time and improved efficiency. Meanwhile, we suggested that 8 mL of 20% KOH methanol solution should be added to perform saponification at 60 degrees C for 30 min. The ratio of solid liquid was 1:8, and the ultrasound-assisted extraction time was 40 min.
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