文献类型： 外文期刊

作者： Xiao, Yan-jia ¹ ; Yu, Si-si ⁴ ; Zheng, Yan-mei ¹ ; Wang, Xin-yue ⁵ ; Zeng, Xiao-yu ⁴ ; Deng, Lan-lan ⁴ ; Li, Han-ren ⁵ ; Zhu, Yong-sheng ¹ ; Cai, Qiu-hua ¹ ; Xie, Hua-an ¹ ; Zhang, Jian-fu ¹ ;

作者机构： 1.Fujian Acad Agr Sci, Rice Res Inst, Fuzhou 350019, Peoples R China

2.Minist Agr & Affairs PR China, Key Lab Germplasm Innovat & Mol Breeding Hybrid Ri, Incubator Natl Key Lab Germplasm Innovat & Mol Bre, Fuzhou 350003, Peoples R China

3.Natl Rice Improvement Ctr China, Minist Sci & Technol, Fujian Engn Lab Crop Mol Breeding, Fujian Key Lab Rice Mol Breeding, Fuzhou 350003, Peoples R China

4.Fujian Agr & Forestry Univ, Agr Coll, Fuzhou 350002, Peoples R China

5.Fujian Agr & Forestry Univ, Plant Protect Coll, Fuzhou 350002, Peoples R China

关键词： Oryza sativa; MATE; Grain size; Grain weight

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：2.2； 五年影响因子：2.5 ）

ISSN： 0006-291X

年卷期： 2024 年 736 卷

页码：

收录情况： SCI

摘要： Multidrug and toxic compound extrusion proteins (MATE) can transport small organic molecules in and out of cells and participate in detoxification, nutrient absorption, disease resistance and plant development processes. These compounds are widely distributed in plants. However, the mechanism by which MATE affects grain development remains elusive. In this study, we studied a MATE transporter, OsMATE2, which localized on the membrane. The CRISPR-Cas9 (CR) knockout line of OsMATE2 presented obvious decreases in grain weight. In addition, root development was also affected. Two proteins that interact with OsMATE2, namely, manganesesuperoxide dismutase (Mn-SOD) and poly(A)-binding protein (PABP), were identified from a screening of yeast library. The results were validated through yeast two-hybrid and bimolecular fluorescence complementation experiments. The CRISPR-Cas9 (CR) knockout lines of Mn-SOD and PABP presented increased grain size and weight. Our findings demonstrated that OsMATE2 interacts with Mn-SOD and PABP to regulate grain development in rice.