文献类型： 外文期刊

作者： Dong, Yan ¹ ; Dong, Yachao ² ; Wu, Ling ⁴ ; Wu, Jianhui ⁵ ; Cheng, Yukun ³ ; Xu, Dengan ⁶ ; Wu, Yuying ² ; Wang, Fengju ² ; Bai, Bin ⁷ ; Wu, Jingchun ² ; Ren, Yan ⁸ ; Hao, Yuanfeng ² ; He, Zhonghu ² ; Chen, Dongsheng ¹ ; Xia, Xianchun ² ;

作者机构： 1.Ningxia Acad Agr & Forestry Sci, Crop Res Inst, 490 Huanghe Rd, Yinchuan 750002, Ningxia, Peoples R China

2.Chinese Acad Agr Sci CAAS, Inst Crop Sci, Natl Wheat Improvement Ctr, 12 Zhongguancun South St, Beijing 100081, Peoples R China

3.Xinjiang Agr Univ, Coll Agron, 311 Nongda East Rd, Urumqi 830052, Xinjiang, Peoples R China

4.Sichuan Acad Agr Sci, Crop Res Inst, 4 Shizishan Rd, Chengdu 610066, Sichuan, Peoples R China

5.Northwest A&F Univ, Coll Agron, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China

6.Qingdao Agr Univ, Coll Agron, 700 Changcheng Rd, Qingdao 266109, Shandong, Peoples R China

7.Gansu Acad Agr Sci, Wheat Res Inst, Lanzhou 730070, Gansu, Peoples R China

8.Henan Agr Univ, Coll Agron, 63 Agr Rd, Zhengzhou 450002, Henan, Peoples R China

9.CAAS, Int Maize & Wheat Improvement Ctr CIMMYT, China Off, Beijing 100081, Peoples R China

关键词： BSE-Seq; BSR-Seq; Genetic mapping; Indel/SNP index; Molecular marker

期刊名称：EUPHYTICA （ 影响因子：1.9； 五年影响因子：2.1 ）

ISSN： 0014-2336

年卷期： 2024 年 220 卷 6 期

页码：

收录情况： SCI

摘要： Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating fungal disease, leading to huge yield losses in wheat production. Identification of resistance genes and closely linked molecular markers can greatly facilitate breeding resistant wheat cultivars. Shaannong69 conferred high resistance to stripe rust both at the seedling and adult-plant stages. The Pst race CYR31 was used to infect Shaannong69, Huixianhong, F1 and F2 plants and F2:3 lines at the seedling stage in the greenhouse. Genetic analysis identified a single dominant gene, designated as YrSN69, conferring resistance to Pst race CYR31 in Shaannong69. Seventy-four of 176 SNPs with the absolute value of triangle SNP-index more than 0.85 were identified in 753.33-766.18 Mb on chromosome 2BL based on bulked segregant RNA sequencing. Fifteen kompetitive allele-specific PCR markers were developed to genotype susceptible F2 plants derived from the Shaannong69/Huixianhong cross. YrSN69 was mapped on chromosome arm 2BL in a 2.0 cM genetic interval, with genetic distances of 0.2 cM and 1.8 cM to markers 2BC17 and 2BA20, respectively, corresponding to a 3.16 Mb physical region based on the IWGSC RefSeq v1.1 with 44 high-confidence annotated genes. The YrSN69 is likely to be a new allele of Yr72 in comparison with known Yr genes on chromosome 2BL. These results provide a solid foundation for map-based cloning of YrSN69 and marker-assisted selection for pyramiding stripe rust resistance genes.