Evaluation of a quantitative real-time PCR for rapid detection of Riemerella Anatipestifer infection in birds
文献类型： 外文期刊
作者： Zhang, Qingshan 1 ; Wan, Chunhe 2 ; Li, Chenxi 1 ; Bai, Xiaofei 1 ; Liu, Ming 1 ; Liu, Siguo 1 ; Zhang, Yun 1 ;
作者机构： 1.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Heilongjiang, Peoples R China
2.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian, Peoples R China
关键词： conventional PCR;TaqMan-based real-time PCR;Riemerella anatipestifer
期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）
ISSN： 0916-7250
年卷期： 2017 年 79 卷 12 期
页码：
收录情况： SCI
摘要： To establish an accurate, rapid, and a quantifiable method for the detection of Riemerella anatipestifer infection, a widespread infectious disease in birds, we developed a TaqMan-based real-time PCR assay by using DtxR gene-specific primers and a TaqMan probe. The standard curve established with a linear correlation (R-2) of 0.998 and efficiency of 99% between the Ct value and the logarithm of the plasmid copy number. The reproducibility and specificity of the real-time PCR assay were confirmed by using plasmids containing DtxR genes or DNAs extracted from well-known bacteria or viruses causing duck diseases. The real-time PCR assay was 100 times more sensitive than the conventional PCR. The results reveal that the established real-time PCR assay might be a useful method for diagnosis and quantitative detection of Riemerella anatipestifer in birds.
- 相关文献
作者其他论文 更多>>
-
Rapid detection of goose megrivirus using TaqMan real-time PCR technology
作者：Fu, Huanru;Chen, Shuyu;Zhang, Jinpeng;Su, Jinbo;Miao, Zhongwei;Huang, Yu;Wan, Chunhe;Fu, Huanru;Chen, Shuyu;Su, Jinbo
关键词：goose megrivirus; TaqMan real-time PCR technology; epidemiological investigation; vertical; transmission
-
Codon usage bias of goose circovirus and its adaptation to host
作者：Xu, Quanming;Cao, Jie;Wan, Chunhe;Rai, Kul Raj;Zhu, Binling;Liu, Dan
关键词：goose circovirus; codon usage bias; natural selection; mutation; host adaptability
-
Rapid detection of pigeon adenovirus 2 using a TaqMan real-time PCR assay
作者：Chen, Cuiteng;Zhu, Chunhua;Chen, Zhen;Cai, Guozhang;Lin, Lin;Zhang, Shizhong;Jiang, Bin;Miao, Zhongwei;Fu, Guanghua;Huang, Yu;Wan, Chunhe
关键词：pigeon adenovirus 2; fiber 2; TaqMan-qPCR assay; YPDS; epidemiological surveillance
-
Genomic analysis reveals the population structure and antimicrobial resistance of avian Pasteurella multocida in China
作者：Jiang, Nansong;Chen, Hongmei;Cheng, Longfei;Fu, Qiuling;Liu, Rongchang;Fu, Guanghua;Wan, Chunhe;Huang, Yu;Jiang, Nansong;Chen, Hongmei;Cheng, Longfei;Fu, Qiuling;Liu, Rongchang;Liang, Qizhang;Fu, Guanghua;Wan, Chunhe;Huang, Yu;Jiang, Nansong;Chen, Hongmei;Cheng, Longfei;Fu, Qiuling;Liu, Rongchang;Liang, Qizhang;Fu, Guanghua;Wan, Chunhe;Huang, Yu
关键词：
-
Simultaneous detection and differentiation of DuCV-1 and DuCV-2 by high-resolution melting analysis
作者：Fu, Huanru;Zhao, Min;Chen, Shuyu;Huang, Yu;Wan, Chunhe;Fu, Huanru;Zhao, Min;Chen, Shuyu
关键词：duck circovirus; DuCV-1; DuCV-2; differentiation; high-resolution melting
-
Hypervirulent fowl adenovirus serotype 4 elicits early innate immune response and promotes virus-induced cellular autophagy in the spleen
作者：Zhu, Chunhua;Zhou, Jiayu;Chen, Zhen;Chen, Cuiteng;Wan, Chunhe;Huang, Yu
关键词：hypervirulent fowl adenovirus serotype 4; TBK1/IRF7 signaling pathway; autophagy; spleen
-
Development of the first officially licensed live attenuated duck hepatitis A virus type 3 vaccine strain HB80 in China and its protective efficacy against DHAV-3 infection in ducks
作者：Fu, Qiuling;Jiao, Wenlong;Liu, Rongchang;Chen, Zhen;Wan, Chunhe;Liang, Qizhang;Shi, Shaohua;Cheng, Longfei;Chen, Hongmei;Jiang, Nansong;Fu, Guanghua;Huang, Yu;Han, Xiangmin;Lai, Zhi;Zhu, Chunhua;Feng, Zhao;Huang, Yaping;Su, Jingliang
关键词：duck hepatitis A virus type 3 (DHAV-3); live attenuated vaccine candidate; genome analysis; protective efficacy
