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Evaluation of a quantitative real-time PCR for rapid detection of Riemerella Anatipestifer infection in birds

文献类型： 外文期刊

作者： Zhang, Qingshan 1 ; Wan, Chunhe 2 ; Li, Chenxi 1 ; Bai, Xiaofei 1 ; Liu, Ming 1 ; Liu, Siguo 1 ; Zhang, Yun 1 ;

作者机构： 1.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Heilongjiang, Peoples R China

2.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian, Peoples R China

关键词： conventional PCR;TaqMan-based real-time PCR;Riemerella anatipestifer

期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）

ISSN： 0916-7250

年卷期： 2017 年 79 卷 12 期

页码：

收录情况： SCI

摘要： To establish an accurate, rapid, and a quantifiable method for the detection of Riemerella anatipestifer infection, a widespread infectious disease in birds, we developed a TaqMan-based real-time PCR assay by using DtxR gene-specific primers and a TaqMan probe. The standard curve established with a linear correlation (R-2) of 0.998 and efficiency of 99% between the Ct value and the logarithm of the plasmid copy number. The reproducibility and specificity of the real-time PCR assay were confirmed by using plasmids containing DtxR genes or DNAs extracted from well-known bacteria or viruses causing duck diseases. The real-time PCR assay was 100 times more sensitive than the conventional PCR. The results reveal that the established real-time PCR assay might be a useful method for diagnosis and quantitative detection of Riemerella anatipestifer in birds.

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[1]Complete Genomic Sequence of the Virulent Bacteriophage RAP44 of Riemerella anatipestifer. Cheng, Long-fei,Chen, Hong-mei,Fu, Guang-hua,Shi, Shao-hua,Wan, Chun-he,Huang, Yu,Cheng, Long-fei,Chen, Hong-mei,Fu, Guang-hua,Shi, Shao-hua,Wan, Chun-he,Huang, Yu,Zheng, Teng. 2012

[2]Immunoproteomic identification of 11 novel immunoreactive proteins of Riemerella anatipestifer serotype 2. Zhai, Zhipeng,Tang, Fang,Lu, Yan,Shao, Jing,Liu, Guangjin,Bao, Yinli,Chen, Mianmian,Shang, Kexin,Fan, Hongjie,Yao, Huochun,Lu, Chengping,Zhang, Wei,Cheng, Longfei.

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