Expression of Wheat High Molecular Weight Glutenin Subunit 1Bx Is Affected by Large Insertions and Deletions Located in the Upstream Flanking Sequences
文献类型： 外文期刊
作者： Geng, Yuke 1 ; Pang, Binshuang 3 ; Hao, Chenyang 1 ; Tang, Saijun 2 ; Zhang, Xueyong 1 ; Li, Tian 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Gene Resources & Germplasm Enhanceme, Minist Agr, Beijing 100193, Peoples R China
2.China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China
3.Beijing Acad Agr & Forestry Sci, Beijing Engn & Tech Res Ctr Hybrid Wheat, Beijing, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2014 年 9 卷 8 期
页码：
收录情况： SCI
摘要： To better understand the transcriptional regulation of high molecular weight glutenin subunit (HMW-GS) expression, we isolated four Glu-1Bx promoters from six wheat cultivars exhibiting diverse protein expression levels. The activities of the diverse Glu-1Bx promoters were tested and compared with beta-glucuronidase (GUS) reporter fusions. Although all the full-length Glu-1Bx promoters showed endosperm-specific activities, the strongest GUS activity was observed with the 1Bx7(OE) promoter in both transient expression assays and stable transgenic rice lines. A 43 bp insertion in the 1Bx7(OE) promoter, which is absent in the 1Bx7 promoter, led to enhanced expression. Analysis of promoter deletion constructs confirmed that a 185 bp MITE (miniature inverted-repeat transposable element) in the 1Bx14 promoter had a weak positive effect on Glu-1Bx expression, and a 54 bp deletion in the 1Bx13 promoter reduced endosperm-specific activity. To investigate the effect of the 43 bp insertion in the 1Bx7(OE) promoter, a functional marker was developed to screen 505 Chinese varieties and 160 European varieties, and only 1Bx7-type varieties harboring the 43 bp insertion in their promoters showed similar overexpression patterns. Hence, the 1Bx7(OE) promoter should be important tool in crop genetic engineering as well as in molecular assisted breeding.
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