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Expression of Cry1Ab Protein in a Marker-Free Transgenic Bt Rice Line and Its Efficacy in Controlling a Target Pest, Chilo suppressalis (Lepidoptera: Crambidae)

文献类型： 外文期刊

作者： Wang, Yanan 1 ; Zhang, Lei 2 ; Li, Yunhe 1 ; Liu, Yanmin 1 ; Han, Lanzhi 1 ; Zhu, Zhen 2 ; Wang, Feng 3 ; Peng, Yufa 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, State Key Lab Plant Genom, Beijing 100101, Peoples R China

3.Fujian Acad Agr Sci, Fujian Prov Key Lab Genet Engn Agr, Fuzhou 350003, Peoples R China

关键词： Bt rice;ELISA;Cry1Ab;pest resistance;bioassay

期刊名称：ENVIRONMENTAL ENTOMOLOGY （ 影响因子：2.377； 五年影响因子：2.298 ）

ISSN： 0046-225X

年卷期： 2014 年 43 卷 2 期

页码：

收录情况： SCI

摘要： A marker-free Bt transgenic rice line, mfb-MH86, was recently developed in China, which contains a cry1Ab gene driven by a ubiquitin promoter. This Bt gene confers resistance to a range of lepidopteran species, including the striped stem borer, Chilo suppressalis (Walker). The expression of Cry1Ab protein in mfb-MH86 leaves, stems and leaf sheaths (hereinafter referred to as stems), and roots was evaluated throughout the rice-growing season using an enzyme-linked immunosorbent assay. In addition, mfb-MH86 resistance to C. suppressalis, a major pest of rice, was evaluated in a laboratory bioassay with field-collected rice stems. Cry1Ab protein levels of mfb-MH86 were highest in leaves (9.71D34.09 mu g/g dry weight [DW]), intermediate in stems (7.66D18.51 mu g/g DW), and lowest in roots (1.95-13.40 mu g/g DW). In all tissues, Cry1Ab levels in mfb-MH86 were higher in seedling and tillering stages than in subsequent growth stages. In the laboratory bioassay, mortality of C. suppressalis after 6 d of feeding on mfb-MH86 stems was 100% throughout the rice-growing season; mortality of C. suppressalis when feeding on stems of the nontransformed isoline, MH86, ranged from 15.0 to 38.3%. The results indicate that Cry1Ab protein levels in mfb-MH86 stems are sufficient to protect plants against C. suppressalis throughout the rice-growing season. Although our results are promising, further comprehensive evaluations of mfb-MH86, including field surveys, will be needed before commercial use.

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