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Identification of fungus-responsive cis-acting element in the promoter of Brassica juncea chitinase gene, BjCHI1

文献类型： 外文期刊

作者： Gao, Ying 1 ; Zan, Xin-Li 1 ; Wu, Xue-Feng 1 ; Yao, Lei 3 ; Chen, Yu-Ling 2 ; Jia, Shuang-Wei 1 ; Zhao, Kai-Jun 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Genet & Breeding, Minist Agr,Natl Key Facil Crop Gene Resources & G, Beijing 100081, Peoples R China

2.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050024, Heibei, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Beijing Agr Biotechnol Res Ctr, Beijing 100097, Peoples R China

关键词： Inducible promoter;cis-Acting element;Fungal elicitor;Transient expression;W-box-like

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2014 年 215 卷

页码：

收录情况： SCI

摘要： Chitinases are a group of pathogenesis-related proteins. The Brassica juncea chitinase gene BjCHI1 is highly inducible by pathogenic fungal infection, suggesting that the promoter of BjCHI1 might contain specific cis-acting element responsive to fungal attack. To identify the fungus-responsive element in BjCHI1 promoter (BjC-P), a series of binary plant transformation vectors were constructed by fusing the BjC-P or its deletion-derivatives to p-glucuronidase (GUS) reporter gene. Expression of the GUS reporter gene was systematically assayed by a transient gene expression system in Nicotiana benthamiana leaves treated with fungal elicitor Hexa-N-Acetyl-Chitohexaose, as well as in transgenic Arabidopsis plants inoculated with fungus Botrytis cinerea. The histochemical and quantitative GUS assays showed that the W-boxlike element (GTAGTGACTCAT) in the region (-668 to -657) was necessary for the fungus-response, although there were another five W-box-like elements in BjC-P. In addition, gain-of-function analysis demonstrated that the fragment (-409 to -337) coupled to the W-box-like element was needed for full magnitude of the fungal induction. These results revealed the existence of a novel regulation mechanism of W-box-like element involved in plant pathogenic resistance, and will benefit the potential application of BjC-P in engineering crops. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

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