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Appressoria Formation in Phytopathogenic Fungi Suppressed by Antimicrobial Peptides and Hybrid Peptides from Black Soldier Flies

文献类型： 外文期刊

作者： Sun, Qianlong 1 ; Zhang, Xin 2 ; Ouyang, Ying 4 ; Yu, Pingzhong 5 ; Man, Yilong 6 ; Guo, Sheng 2 ; Liu, Sizhen 2 ; Chen, Yue 2 ; Wang, Yunsheng 1 ; Tan, Xinqiu 2 ;

作者机构： 1.Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China

2.Inst Plant Protect, Hunan Acad Agr Sci, Changsha 410125, Peoples R China

3.Hunan Univ, Coll Biol, Longping Branch, Changsha 410125, Peoples R China

4.Coll Plant Sci, Hunan Biol & Electromech Polytech, Changsha 410127, Peoples R China

5.Inst Plant Protect, Beijing Acad Agr & Forestry Sci, Beijing 100097, Peoples R China

6.Agr Biotechnol Inst, Hunan Acad Agr Sci, Changsha 410125, Peoples R China

关键词： AMPs; hybrid peptide; Colletotrichum acutatum; Magnaporthe oryzae; Hermetia illucens

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2023 年 14 卷 5 期

页码：

收录情况： SCI

摘要： Antimicrobial peptides (AMPs) from black solider flies (Hermetia illucens, BSF) exhibiting broad-spectrum antimicrobial activity are the most promising green substitutes for preventing the infection of phytopathogenic fungi; therefore, AMPs have been a focal topic of research. Recently, many studies have focused on the antibacterial activities of BSF AMPs against animal pathogens; however, currently, their antifungal activities against phytopathogenic fungi remain unclear. In this study, 7 AMPs selected from 34 predicted AMPs based on BSF metagenomics were artificially synthesized. When conidia from the hemibiotrophic phytopathogenic fungi Magnaporthe oryzae and Colletotrichum acutatum were treated with the selected AMPs, three selected AMPs-CAD1, CAD5, and CAD7-showed high appressorium formation inhibited by lengthened germ tubes. Additionally, the MIC50 concentrations of the inhibited appressorium formations were 40 mu M, 43 mu M, and 43 mu M for M. oryzae, while 51 mu M, 49 mu M, and 44 mu M were observed for C. acutatum, respectively. A tandem hybrid AMP named CAD-Con comprising CAD1, CAD5, and CAD7 significantly enhanced antifungal activities, and the MIC50 concentrations against M. oryzae and C. acutatum were 15 mu M and 22 mu M, respectively. In comparison with the wild type, they were both significantly reduced in terms of virulence when infection assays were performed using the treated conidia of M. oryzae or C. acutatum by CAD1, CAD5, CAD7, or CAD-Con. Meanwhile, their expression levels of CAD1, CAD5, and CAD7 could also be activated and significantly increased after the BSF larvae were treated with the conidia of M. oryzae or C. acutatum, respectively. To our knowledge, the antifungal activities of BSF AMPs against plant pathogenic fungi, which help us to seek potential AMPs with antifungal activities, provide proof of the effectiveness of green control strategies for crop production.

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