您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular characterization and functional analysis of two new-1,4-endoglucanase genes (Ha-eng-2, Ha-eng-3) from the cereal cyst nematode Heterodera avenae

文献类型： 外文期刊

作者： Long, H. 2 ; Peng, D. 1 ; Huang, W. 1 ; Peng, H. 1 ; Wang, G. 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.Chinese Acad Trop Agr Sci, Key Lab Pests Comprehens Governance Trop Crops, Minist Agr, Environm & Plant Protect Inst, Danzhou 571737, Peoples R China

关键词： cell wall degradation;cellulose;Heterodera avenae;RNA

期刊名称：PLANT PATHOLOGY （ 影响因子：2.59； 五年影响因子：2.924 ）

ISSN： 0032-0862

年卷期： 2013 年 62 卷 4 期

页码：

收录情况： SCI

摘要： Parasitism genes encoding secretory proteins expressed in the pharyngeal glands of plant-parasitic nematodes play a crucial role in nematode parasitism of plants. Two new -1,4-endoglucanase genes (Ha-eng-2 and Ha-eng-3) expressed in the pharyngeal glands of the sedentary cyst nematode, Heterodera avenae, were cloned. Both of the predicted proteins have a putative signal peptide for secretion and a catalytic domain. Neither peptide linkers nor cellulose binding domains were present. In situ hybridization showed that the transcripts of Ha-eng-2 and Ha-eng-3 accumulated specifically in the two subventral gland cells of H.avenae. RT-PCR analysis confirmed that their transcriptions were strong in the preparasitic and early parasitic second-stage juveniles, and were undetectable at the late parasitic stages of the nematode. Cellulase activities of the recombinant proteins HA-ENG-2 and HA-ENG-3 were confirmed in vitro. Knocking down Ha-eng-2 using RNA interference reduced nematode infectivity by 40%. The results indicate that these -1,4-endoglucanases can be secreted into plant tissues and play an important role in the wall degradation of plant cells during penetration and the migration of second-stage juveniles in host roots.

  • 相关文献

[1]Host status of Brachypodium distachyon to the cereal cyst nematode. Chen Chang-long,Liu Shu-sen,Liu Qian,Liu Pei,Zhao Jian-long,Jian Heng,Chen Chang-long,Li Hong-jie,Liu Shu-sen,Niu Jun-hai,Liu Zhi-yong,Liu Zhi-yong. 2018

[2]甘蔗茎RNA提取方法研究(摘要)(英文). 陈萍,罗挺,蔡文伟. 2010

[3]甘蔗茎RNA提取方法研究. 陈萍,罗挺,蔡文伟. 2010

[4]改良一步法提取植物RNA·DNA和蛋白质的研究. 肖笑,韩斌,徐晟,李乐,何健,黄明,谢贵水,沈文飚. 2011

[5]一种适用于多糖多酚植物的高质量RNA快速提取方法. 阮孟斌,李文彬,于晓玲,彭明. 2011

[6]改良一步法提取植物RNA、DNA和蛋白质的研究(英文). 肖笑,韩斌,徐晟,李乐,何健,黄明,谢贵水,沈文飚. 2010

[7]香蕉果实RNA提取方法的改进和比较. 李燕强,金志强,徐碧玉. 2005

[8]一种适合油棕不同组织RNA提取的方法. 李静,王永,杨耀东,雷新涛,肖勇,夏薇. 2014

[9]非编码RNA及其研究进展. 秦云霞,田娥,刘志昕,曾华金. 2004

[10]柱花草叶片组织总RNA提取方法. 申建斌,田维敏,蒋昌顺. 2007

[11]一种简单高效的真菌总RNA提取方法. 淡明,黄海波,郭安平,贺立卡. 2006

[12]Comparison of Structural Modification of Sugarcane Bagasse Cellulose Regenerated from Three Imidazolium-Based Ionic Liquids. Cui, Lihong,Wei, Xiaoyi,Fu, Tiaokun,Chang, Gang,Feng, Qin,Tang, Bing. 2017

[13]Preparation and Characterization of Fe3O4/Cellulose Composite Material by the Method of Solidoid Compound. Xia, Wen,Yin, Chan,Wang, Fei,Li, Ji-hua,Wei, Xiao-yi,Fu, Tiao-kun. 2016

[14]Comparative Study of Three Pretreatment Methods on Sugarcane Bagasse. Cui, Lihong,Li, Jihua,Wei, Xiaoyi,Chang, Gang,Tang, Bing,Wu, Hao. 2017

[15]Optimization of Enzymatic Process for Vanillin Extraction Using Response Surface Methodology. Gu, Fenglin,Xu, Fei,Tan, Lehe,Wu, Huasong,Chu, Zhong,Wang, Qinghuang,Gu, Fenglin,Xu, Fei,Tan, Lehe,Wu, Huasong,Chu, Zhong,Wang, Qinghuang,Gu, Fenglin,Xu, Fei,Tan, Lehe,Wu, Huasong,Chu, Zhong,Wang, Qinghuang. 2012

[16]Preparation of pH- and salinity-responsive cellulose copolymer in ionic liquid. Wei, Xiaoyi,Chang, Gang,Li, Jihua,Wang, Fei,Cui, Lihong,Fu, Tiaokun,Kong, Lingxue.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台