Characterization of myostatin gene (MSTN) of Pekin duck and the association of its polymorphism with breast muscle traits
文献类型: 外文期刊
作者: Xu, T. S. 1 ; Gu, L. H. 1 ; Zhang, X. H. 4 ; Ye, B. G. 5 ; Liu, X. L. 1 ; Hou, S. S. 3 ;
作者机构: 1.Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling, Shaanxi, Peoples R China
2.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Res Inst, Danzhou, Hainan, Peoples R China
3.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
4.Henan Univ Sci & Technol, Coll Anim Sci, Luoyang, Henan, Peoples R China
5.Hainan Acad Agr Sci, Inst Anim Sci & Vet, Haikou, Hainan, Peoples R China
关键词: Pekin duck;Breast muscle;MSTN;Gene expression
期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )
ISSN: 1676-5680
年卷期: 2013 年 12 卷 3 期
页码:
收录情况: SCI
摘要: Myostatin, encoded by the MSTN gene, is a negative regulator of muscle growth, and its expression level in muscle tissue is closely correlated with muscle growth and satellite cell proliferation. To identify the characteristics of the Pekin duck MSTN gene and the relationship between its polymorphism and breast muscle traits in Pekin duck, cDNA cloning and analysis and the expression pattern in breast muscle development and polymorphism were performed using molecular cloning, quantitative real-time reverse-transcription polymerase chain reaction, and molecular marker technology. The results showed that a 1320-bp sequence, including a 93-bp 5'-UTR, 1128-bp CDS, and 99-bp 3'-UTR, was obtained, and two alternative splicing isoforms were detected. The alternative splicing isoforms encoded 375- and 251-amino acid residues. The amino acid sequence of Pekin duck MSTN was similar to other vertebrates and exhibited the highest similarity to chicken. The expression pattern of MSTN in breast muscle tissue showed a tendency to increase, except for a slight decrease at 6 weeks. Three single nucleotide polymorphisms were found in the Pekin duck MSTN gene by cDNA sequencing from different individuals. The T129C had significant association with breast muscle thickness, and the T952C had significant association with the fossilia ossis mastodi length. This study reveals the molecular characteristics of the Pekin duck MSTN gene and the relationship of its polymorphism with breast muscle traits in Pekin duck. Therefore, it can provide some useful basic understanding of MSTN functions.
- 相关文献
作者其他论文 更多>>
-
Characterization of MUSTN1 gene and its relationship with skeletal muscle development at postnatal stages in Pekin ducks
作者:Xu, T. S.;Gu, L. H.;Liu, X. L.;Xu, T. S.;Xu, T. S.;Hou, S. S.;Zhang, X. H.;Sun, Y.;Ye, B. G.
关键词:MUSTN1;Pekin ducks;Skeletal muscle development;Molecular characteristics;Expression profiles
-
The Genetic Diversity of Hainan Black Goat by Capillary Gel Electrophoresis with Microsatellite Markers in Tropical Area of China
作者:Zhou, H. L.;Guan, S.;Hou, G. Y.;Wang, D. J.;Xu, T. S.;Wang, D. F.;Shi, L. G.;Rong, G.;Li, M.
关键词:Genetic diversity;Hainan Black goat;capillary gel electrophoresis;microsatellite markers;heterozygotes;China
-
Estimates of Genetic Parameters for Body Weight and Carcass Composition in Pekin Ducks
作者:Xu, T. S.;Huang, W.;Hou, S. S.;Xu, T. S.;Liu, X. L.;Xu, T. S.
关键词:Pekin duck;genetic parameter;body weight;body size;carcass composition;correlation
-
The Prophet of PIT1 Gene Variation and its Effect on Growth Traits in Chinese Indigeous Goat
作者:Xu, T. S.;Zhou, H. L.;Liu, J. B.;Yao, D. W.;Cai, H. F.;Chen, H.;Lan, X. Y.
关键词:Goat;Prophet of PIT1 (PROP1) gene;Single Nucleotide Polymorphism (SNP);PCR-RFLP;Marker Assisted Selection (MAS);genotype
-
Arginine affects appetite via nitric oxide in ducks
作者:Wang, C.;Hou, S. S.;Huang, W.;Rong, G. H.;Xie, M.;Wang, C.;Xu, T. S.
关键词:duck;arginine;appetite;nitric oxide