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Identification of glutinous maize landraces and inbred lines with altered transcription of waxy gene

文献类型： 外文期刊

作者： Bao, Jian-dong 1 ; Yao, Jian-qiang 3 ; Zhu, Jin-qing 3 ; Hu, Wei-min 1 ; Cai, Da-guang 4 ; Li, Yu 5 ; Shu, Qing-yao 6 ; F 1 ;

作者机构： 1.Zhejiang Univ, Dept Agron, Hangzhou 310058, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Crop Sci, Hangzhou 310021, Zhejiang, Peoples R China

4.Univ Kiel, Dept Mol Phytopathol, D-24118 Kiel, Germany

5.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China

6.Zhejiang Univ, Inst Nucl Agr Sci, Hangzhou 310029, Zhejiang, Peoples R China

关键词： Granule-bound starch synthase;Wx mutation;Alternative splicing;Chinese waxy maize

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2012 年 30 卷 4 期

页码：

收录情况： SCI

摘要： Waxy maize has little or very low content of amylose (< 5 %) in grain starch and carries null mutations of the waxy (Wx) gene. With important uses as fresh maize or as an ingredient in food, textile, adhesive, and paper industries, two types of wx allele, wx-D7 and wx-D10, had been identified. In the present study, 10 accessions carrying neither wx-D7 nor wx-D10 allele were identified from a collection of 325 waxy maize accessions. The Wx locus of these 10 accessions was sequenced, but no potential causative mutation was detected. Further sequencing of the full-length complementary DNAs revealed that 6 of the 10 accessions had alternative splicing patterns while the other 4 had wild-type Wx transcripts. Among the six accessions, one generated transcripts identical to the wx-D7 allele, two had the same transcripts as the wx-D10 allele, another two generated transcripts with deletion of the 10th and the 11th exon, yet the other one produced transcripts of various lengths due to deletions from part of the 2nd exon to part of the 12th exon. The wx alleles with the above alternative splicing modes are referred to as wx-tD7, wx-tD10, wx-tD10-11, and wx-tD2-12, respectively. Real-time quantitative reverse-transcription polymerase chain reaction analysis of two waxy accessions that produced Wx transcripts showed significantly decreased expression, having only 47.3 and 3.6 % transcription level compared with B73. Sequence analysis of deletions in the transcripts with comparison with wild one showed short direct repeats at deletion endpoints, similar to reported signatures of DNA deletions. The waxy accessions present unique wx alleles for waxy maize breeding as well as for transcriptional regulation studies in plants.

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