Identification of in vivo interaction between rabbit hemorrhagic disease virus capsid protein and minor structural protein
文献类型： 外文期刊
作者： Yang ZongWei 1 ; Ni Zheng 2 ; Yun Tao 2 ; Chen ZongYan 1 ; Li ChuanFeng 1 ; Liu GuangQing 1 ;
作者机构： 1.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China
2.Zhejiang Acad Agr Sci, Hangzhou 300021, Zhejiang, Peoples R China
3.Gansu Agr Univ, Fac Vet Med, Lanzhou 730070, Peoples R China
关键词： rabbit hemorrhagic disease virus;VP60;VP10;protein-protein interaction
期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）
ISSN： 1001-6538
年卷期： 2012 年 57 卷 30 期
页码：
收录情况： SCI
摘要： We investigated the ability of the rabbit hemorrhagic disease virus (RHDV) capsid protein (VP60) to interact specifically with the minor structural protein VP10, using an in vivo cell-based CheckMate (TM) Mammalian Two-Hybrid System. RHDV VP60 protein interacted specifically with VP10. Immunofluorescence analysis and co-immunoprecipitation with specific antibodies revealed the existence of biologically important VP60/VP10 complexes. However, when VP60 was divided into two fragments, the interaction between VP60 and VP10 was impaired dramatically. These results will be helpful for further investigating the mechanism of RHDV particle assembly.
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