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Editing of eIF(iso)4E.c confers resistance against Turnip mosaic virus in Brassica rapa

文献类型： 外文期刊

作者： Liu, Yujia 1 ; Xin, Xiaoyun 1 ; Li, Peirong 1 ; Wang, Weihong 1 ; Yu, Yangjun 1 ; Zhao, Xiuyun 1 ; Zhang, Deshuang 1 ; Wang, Jiao 2 ; Zhang, Fenglan 1 ; Zhang, Shujiang 3 ; Yu, Shuancang 1 ; Su, Tongbing 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, State Key Lab Vegetable Biobreeding, Beijing 100097, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing Key Lab Vegetable Germplasms Improvement, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China

3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

关键词： Brassica rapa; eIF(iso)4E.c; TuMV; Ribo-seq

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：5.5 ）

ISSN： 2095-9885

年卷期： 2024 年 10 卷 4 期

页码：

收录情况： SCI

摘要： Turnip mosaic virus (TuMV) constitutes one of the primary diseases affecting Brassica rapa, severely impacting its production and resulting in crop failures in various regions worldwide. Recent research has demonstrated the significance of plant translation initiation factors, specifically the eIF4E and eIF4G family genes, as essential recessive disease resistance genes. In our study, we conducted evolutionary and gene expression studies, leading us to identify eIF(iso)4E.c as a potential TuMV-resistant gene. Leveraging CRISPR/Cas9 technology, we obtained mutant B. rapa plants with edited eIF(iso)4E.c gene. We confirmed eIF(iso)4E.c confers resistance against TuMV through phenotypic observations and virus content evaluations. Furthermore, we employed ribosome profiling assays on eif(iso)4e.c mutant seedlings to unravel the translation landscape in response to TuMV. Interestingly, we observed a moderate correlation between the fold changes in gene expression at the transcriptional and translational levels (R2 1/4 0.729). Comparative analysis of ribosome profiling and RNA-seq data revealed that plant-pathogen interaction, and MAPK signaling pathway-plant pathways were involved in eIF(iso)4E.c-mediated TuMV resistance. Further analysis revealed that sequence features, coding sequence length, and normalized minimal free energy, influenced the translation efficiency of genes. Our study highlights that the loss of eIF(iso) 4E.c can result in a highly intricate translation mechanism, acting synergistically with transcription to confer resistance against TuMV.

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