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Embryonic growth and effect of embryonic age on quantitative and functional characteristics of duck primary hepatocytes

文献类型： 外文期刊

作者： Wei, Jie 1 ; Zhang, Bo 1 ; Tang, Jing 1 ; Cao, Junting 1 ; Du, Chenchen 1 ; Wang, Zhen 1 ; Zhang, Yunsheng 1 ; Xie, Ming 1 ; Zhou, Zhengkui 1 ; Hou, Shuisheng 1 ;

作者机构： 1.Chinese Acad Agr Sci, Key Lab Anim Poultry Genet Breeding & Reprod, Minist Agr & Rural Affairs, State Key Lab Anim Nutr & Feeding,Inst Anim Sci, Beijing 100193, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing 100097, Peoples R China

3.Chinese Acad Agr Sci, Inst Feed Res, Beijing 100081, Peoples R China

关键词： Duck; embryo and liver; primary hepatocyte; embryonic age; quantitative and functional characteristic

期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 4 期

页码：

收录情况： SCI

摘要： Primary hepatocytes (PH) have been widely used in metabolic and disease -resistance mechanism research. However, hepatocyte isolation (HI) remains challenging in ducks. This study aimed to explore embryonic growth and the effect of embryonic age (EA) on the quantitative and functional characteristics of PH in ducks. For embryonic growth, the size and weight of the embryo and liver were determined from 6 to 28 EA (E6-E28, similar below). As EA increased, the corresponding size and weight grew significantly. Specifically, embryonic length varied from 12.5 mm to 133.0 mm, and liver width varied from 2.0 mm to 26.2 mm. Embryonic weight ranged from 0.259 g to 53.58 g, and liver weight ranged from 0.007 g to 1.765 g. Liver index initially decreased and then increased with a ratio ranging from 1.06 to 3.29%. For quantitative and functional characteristics, they were determined from E6 to E22, as there were no obvious liver features before E6 and few cells obtained after E22. The number of cells isolated in liver increased from E6 to E16 and then sharply decreased from E16 to E22. The viability remained relatively stable from E6 to E10 and then decreased from E12 to E22. The comprehensive intensity of hepatic glycogen was stronger at E8 and E14. Albumin expression increased markedly from E6 to E18 by qPCR, and the overall albumin expression was stronger at E8 and E14 by immunofluorescence assay. Hepatocyte purity exceeded 90% except for E20 and E22. During culture, cell clusters appeared after 24-h culture, which were identified as nonhepatocytes. The growth curve showed an initial increase in cell quantity followed by a decrease, another increase, and then remaining stable. In conclusion, EA had a significant effect on the quantitative and functional characteristics of PH, and the suitable EA for HI were E8 and E14. Considering better operability and quantity, E14 was the optimal EA, laying a solid foundation for further hepatocyte purification, nutrient metabolism, and disease -resistance mechanism explorations in ducks.

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