文献类型： 外文期刊

作者： Liu, Jing ¹ ; Wang, Zhengfeng ² ; Chen, Zhenjiang ¹ ; White, James F. ³ ; Malik, Kamran ¹ ; Chen, Taixiang ¹ ; Li, Chunjie ¹ ;

作者机构： 1.Lanzhou Univ, Minist Educ,Engn Res Ctr Grassland Ind,Gansu Tech, Minist Agr & Rural Affairs,State Key Lab Grasslan, Ctr Grassland Microbiome,Coll Pastoral Agr Sci &, Lanzhou 730020, Peoples R China

2.Gansu Acad Agr Sci, Econ Crops & Malt Barley Res Inst, Lanzhou 730070, Peoples R China

3.Rutgers State Univ, Dept Plant Biol, New Brunswick, NJ 08901 USA

4.Chinese Acad Forestry Sci, Grassland Res Ctr Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China

关键词： Epichloe bromicola; novel symbionts; peramine; nutritional properties; 16s rRNA; ITS rDNA

期刊名称：JOURNAL OF FUNGI （ 影响因子：5.724； 五年影响因子：6.413 ）

ISSN：

年卷期： 2022 年 8 卷 2 期

页码：

收录情况： SCI

摘要： Hordeum vulgare is an important source of feed and forage for livestock, and of food and drink for humans, but its utilization rate is lower than that of other cereal crops, thus it is crucial to improve barley agronomic traits and production. Epichloe bromicola is an endophyte that was isolated from wild barley (Hordeum brevisubulatum). Previous studies have found that Epichloe can indirectly influence the growth of host plants by affecting soil chemical characteristics, the microbial community, and by producing a range of secondary metabolites. However, underlying effects of Epichloe on the abundance and diversity of soil and root microbes have not been well-studied. In addition, there is a question regarding the relationship between endophyte-produced alkaloids and effects on the root and rhizosphere microbial communities. The objective of this study was to investigate changes in agronomic traits, nutritional properties, peramine, soil chemical and microbial community in the fourth generation of new barley symbionts EI (E. bromicola-infection) and EF (E. bromicola-free) in LQ+4 and LZ+4. We understand the plant height and biomass of EI in LZ+4 were significantly higher than those of EF. The HPLC analysis showed that the peramine content of EI in LQ+4 and LZ+4 was 0.085 and 0.1 mg/g, respectively. We compared the bacterial and fungal communities by analyzing the 16s rRNA (for bacteria) and ITS rDNA regions (for fungi). Our data revealed that the composition of fungal communities in rhizosphere soil of LZ+4 EI are higher than EF. In addition, the diversity and richness of fungal communities in root and rhizosphere soil of LQ+4 EI and LZ+4 EI are significantly higher than EF. Rhizosphere soil microbial community composition was higher than that in roots in LQ+4 and LZ+4. Peramine was significantly and positively correlated with the richness of the soil fungal community. Moreover, the principal component analysis (PCoA) results indicated that E. bromicola significantly influenced the community composition of root and rhizosphere soil microbes in both LQ+4 and LZ+4. Our results illustrate that E. bromicola can influence barley growth, peramine production and microbial communities associated with barley.