Generation of infectious and pathogenic duck hepatitis virus type 1 from cloned full-length cDNA
文献类型： 外文期刊
作者： Yun, T. 2 ; Ni, Z. 3 ; Liu, G. Q. 1 ; Yu, B. 3 ; Chen, L. 3 ; Huang, J. G. 3 ; Zhang, Y. M. 2 ; Chen, J. P. 3 ;
作者机构： 1.Chinese Acad Agr Sci, Dept Avian Infect Dis, Shanghai Vet Res Inst, Key Lab Anim Parasitol,Chines Minist Agr, Shanghai 200241, Peoples R China
2.NW A&F Univ, Coll Vet Med, Yongling 712100, Shaanxi, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China
关键词： RNA viruses;Picornaviruses;Infectious cDNA;Duck hepatitis virus type 1
期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）
ISSN： 0168-1702
年卷期： 2010 年 147 卷 2 期
页码：
收录情况： SCI
摘要： A full-length cDNA clone of duck hepatitis virus type 1 (DHV-1) isolated from duck was assembled in the plasmid vector pBluescript II SK+. RNA synthesized in vitro by means of a Sp6 promoter inserted in front of the cDNA produced infectious particles after transfection of BHK-21 cells, as shown by the appearance of cytopathic effect. The rescued virus was also found to be highly pathogenic to young ducks by intradermal injection and resulted in a fatal disease indistinguishable from that of wild-type virus. The availability of the first cDNA clone of DHV-1 will allow examination of the molecular mechanisms behind DHV-1 virulence and attenuation, which could in turn lead to the production of second-generation, genetically engineered DHV-1 vaccines. (C) 2009 Elsevier B.V. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
Genome-wide association study identifies variants associated with semen volume in white-feathered broilers
作者：Zhang, G. M.;Liu, P. H.;Zheng, J. M.;Zhao, G. P.;Xing, W. H.;Wen, J.;Li, Q. H.;Chen, L.
关键词：GWAS; semen volume; white-feathered broiler
-
Cloning, expression and bioinformatics analysis of a putative pigeon melanoma differentiation-associated gene 5
作者：Li, G. -Q.;Tian, Y.;Chen, L.;Shen, J. -D.;Tao, Z. -R.;Zeng, T.;Xu, J.;Lu, L. -Z.;Li, G. -Q.;Tian, Y.;Zeng, T.;Lu, L. -Z.
关键词：Bioinformatics; gene expression; melanoma differentiation-associated gene 5; pigeon; structure
-
Molecular cloning, characterisation, and expression patterns of pigeon CCAAT/enhancer binding protein-alpha and -beta genes
作者：Tian, Y.;Li, G.;Shen, J.;Tao, Z.;Chen, L.;Zeng, T.;Lu, L.;Tian, Y.;Li, G.;Zeng, T.;Lu, L.
关键词：Bioinformatics analysis; C; EBP-alpha; C; EBP-beta; expression profile; pigeon
-
Genetic parameters of feed efficiency traits and their relationships with egg quality traits in laying period of ducks
作者：Zeng, T.;Chen, L.;Tian, Y.;Shen, J.;Lu, L.;Zhang, H.;Liu, J.
关键词：RFI;feed efficiency;genetic parameter;egg quality;laying duck
-
Gene Expression Patterns of Geese Expression Patterns of L-Fabp, Spot 14, Ob and Apo A1 Genes in Different Tissues of Overfed and Control Geese
作者：Ma, Y. F.;Chen, L.;He, J.;Tian, Y.;Xu, X. Q.;Du, X.;Lu, L. Z.
关键词：Langde goose;lipids related genes;gene expression;fatty liver
-
Gene expression profiling reveals candidate genes related to residual feed intake in duodenum of laying ducks
作者：Zeng, T.;Huang, L.;Ren, J.;Chen, L.;Tian, Y.;Lu, L.;Huang, L.;Huang, Y.;Zhang, H.;Du, J.
关键词：duck;duodenum;gene expression;metabolism;residual feed intake
-
An effective route for the micropropagation of Medinilla formosana through ovary culture in vitro
作者：Wang, Y.;Feng, D. D.;Chen, J. P.;Li, X. B.
关键词：Medinilla;ovary culture;browning;inhibitor;micropropagation
