文献类型： 外文期刊

作者： Liu Jian-xin ¹ ; Yu Chun-ming ¹ ; Tang Shou-wei ¹ ; Zhu Ai-guo ¹ ; Wang Yan-zhou ¹ ; Zhu Si-yuan ¹ ; Ma Xiong-feng ¹ ; Xi ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 420105, Hunan, Peoples R China

2.Zhejiang Acad Agr Sci, Cotton & Fibre Res Inst Xiaoshan, Hangzhou 311202, Zhejiang, Peoples R China

关键词： clone;GalAT;pectin;ramie;real-time quantitative PCR analysis

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2009 年 8 卷 6 期

页码：

收录情况： SCI

摘要： To isolate the cDNA partial sequence of key enzyme gene GalAT for pectin biosynthesis in ramie [Boehmeria nivea (L.) Gaud], and thus to understand the expression of GalAT gene in different tissues of ramie, degenerate primer was designed according to GalAT conserved sequence in other species reported, and the cDNA sequence of GalAT gene from ramie variety Zhongzhu 1 was cloned by RT-PCR method based on the degenerate primer. The cDNA revealed a 986-bp in length which encoded 328 amino acids. The cDNA sequence and putative amino acid sequence of GalAT shared high identity with previously reported Arabidopsis thaliana GAUT4 (GalAT) as 77 and 83%, respectively. Molecular evolution analysis showed that the putative amino acid sequence and Arabidopsis thaliana GAUT4 gathered to a same group. Real-time quantitative PCR analysis showed that GalAT mRNA accumulated most abundantly in root, and GalAT transcripts in all kinds of ramie tissues in turn revealed as follows: root > leaf> bast> or approximate to xylem.