文献类型： 外文期刊

作者： Lai, Gongti ¹ ; He, Liyuan ¹ ; Lin, Junxuan ¹ ; Guo, Aolin ¹ ; Que, Qiuxia ¹ ; Che, Jianmei ² ; Guan, Xuefang ¹ ; Xiang, Jiang ³ ; Lai, Chengchun ¹ ;

作者机构： 1.Fujian Acad Agr Sci, Inst Food Sci & Technol, Fuzhou 350003, Fujian, Peoples R China

2.Fujian Acad Agr Sci, Inst Resources Environm & Soil Fertilizer, Fuzhou 350003, Fujian, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China

4.Minist Agr & Rural Affairs, Coconstruct Minist & Prov, Key Lab Subtrop Characterist Fruits Vegetables & E, Fuzhou 350003, Fujian, Peoples R China

5.Fujian Key Lab Agr Prod Food Proc, Fuzhou 350003, Fujian, Peoples R China

关键词： Vitis davidii; Anthocyanin; UDP-Glycose: flavonoid glycosyltransferase; (UFGT); Natural products; Plant cell factory

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.9； 五年影响因子：6.1 ）

ISSN： 2212-4292

年卷期： 2024 年 62 卷

页码：

收录情况： SCI

摘要： Anthocyanins are a type of plant natural products (PNPs) that play a crucial role in various fields, including food, medicine, cosmetics, and dyes. However, their primary source is plant extraction, leading to significant ecological damage and resource wastage issues. To address the challenge of achieving ecologically sustainable high-throughput anthocyanin production, this study proposes a plant cell factory (PCF) strategy based on suspension cell culture (SCC). In this study, the key gene VdUFGT involved in anthocyanin biosynthesis was cloned from Vitis davidii, and gene overexpression was conducted using Agrobacterium-mediated methods. Notably, the vgt3 transgenic cell line exhibited 4.4-fold increase in anthocyanin content, reaching 286.9 mu g/g (FW) in solid medium, along with enhanced levels of flavonoids and proanthocyanidins. Integrative analysis of transcriptomics and metabolomics demonstrated that VdUFGT significantly promoted the expression of genes involved in anthocyanin biosynthesis, leading to the enhanced accumulation of various flavonoids, including anthocyanins. A small-scale plant cell factory was established, achieving an impressive production of 4.11 mg/g (DW) of anthocyanin within just 12 days, which is 8.5 times higher than that of the WT cell line, with the yield of purified freeze-dried anthocyanin powder reaching 3.17%. The plant cell factory, employing genetic engineering and suspension cell culture, offers a safe, ecological, economical, and efficient approach for the high-throughput production of plant natural products such as anthocyanins.