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Tire rubber-derived contaminant 6PPD had the potential to induce metabolism disorder in early developmental stage of zebrafish

文献类型： 外文期刊

作者： Fang, Chanlin 1 ; Di, Shanshan 2 ; Wang, Caihong 1 ; Hu, Qinglian 1 ; Jin, Yuanxiang 1 ;

作者机构： 1.Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Hangzhou 310032, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Key Lab Detect Pesticide Residues & Control Zhejia, Hangzhou 310021, Peoples R China

3.Chaowang Rd 18, Hangzhou 310014, Zhejiang, Peoples R China

关键词： 6PPD; Non-targeted metabolomics; Metabolism disorder; Larval zebrafish

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY （ 影响因子：4.3； 五年影响因子：4.1 ）

ISSN： 1532-0456

年卷期： 2025 年 287 卷

页码：

收录情况： SCI

摘要： The increasing release of tire-derived particles, particularly those containing N-(1,3-dimethylbutyl)-N '-phenyl-pphenylenediamine (6PPD), into the environment has raised concerns regarding their ecological impact. This study aims to elucidate the toxicological effects of 6PPD on the metabolism in early developmental stage of zebrafish. Larval zebrafish were exposed to 10 and 100 mu g/L 6PPD, and some endpoints in biochemical parameters, gene expression, and metabolism were analyzed. The results showed that 6PPD exposure disrupted glucolipid metabolism in zebrafish larvae, evidenced by increased triglyceride (TG) levels and decreased glucose content. Nile red staining indicated significant lipid accumulation in the liver and intestines. Additionally, RTqPCR analysis revealed the upregulation of genes involved in lipid synthesis and metabolism, such as ppar-gamma and fas, and downregulation of glycolysis-related genes like pk and gk. Furthermore, the untargeted metabolomics technique was used to identify a total of 220 differentially expressed metabolites (DEMs) with changes in amino acid metabolism, lipid metabolism, and the TCA cycle. KEGG pathway enrichment analysis highlighted disruptions mainly in Taurine and hypotaurine metabolism, Arginine and proline metabolism, and Histidine metabolism, which played very important roles on energy metabolism in zebrafish. The results provided some critical insights into the ecological risks associated with 6PPD.

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