Molecular identification of Trichogramma species from South and South-East Asia and natural Wolbachia infection
文献类型： 外文期刊
作者：
Liu, Yu-Di
1
;
Hou, Mao-Lin
1
;
Song, Kai
2
;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, 2 West Yuan Ming Yuan Rd, Beijing 100193, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Dryland Farming Inst, Hengshui, Peoples R China
期刊名称：ENTOMOLOGICA FENNICA （ 影响因子：0.767； 五年影响因子：0.755 ）
ISSN： 0785-8760
年卷期： 2017 年 28 卷 2 期
页码：
收录情况： SCI
摘要： Trichogramma wasps were collected from the parasitized eggs of lepidopteran pests from 21 sampling sites in East Asia and South-East Asia. Six Trichogramma species were identified based on the molecular identification method using the internal transcribed spacer 2 (ITS2) region of the rDNA of Trichogramma chilonis, T evanescens, T ostriniae, T embryophagum, T dendrolimi and T japonicum. The results of molecular identification were confirmed by morphological identification. Additionally, natural populations were screened for the prevalence of Wolbachia. Five out of 21 populations were infected by the same Wolbachia strain, which was identified by using Wolbachia wsp gene and multi locus sequencing approach. The phylogenetic analysis of Wolbachia wsp sequences revealed that the Wolbachia strain was classified in the strain wEvaA in the group of EvA of the supergroup A.
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