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Selection of relatively exact reference genes for gene expression studies in goosegrass (Eleusine indica) under herbicide stress

文献类型： 外文期刊

作者： Chen, Jingchao 1 ; Huang, Zhaofeng 1 ; Huang, Hongjuan 1 ; Wei, Shouhui 1 ; Liu, Yan 2 ; Jiang, Cuilan 1 ; Zhang, Jie; 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, Key Lab Weed & Rodent Biol & Management, Beijing 100193, Peoples R China

2.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Danzhou 571737, Peoples R China

3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Goosegrass (Eleusine indica) is one of the most serious annual grassy weeds worldwide, and its evolved herbicide-resistant populations are more difficult to control. Quantitative real-time PCR (qPCR) is a common technique for investigating the resistance mechanism; however, there is as yet no report on the systematic selection of stable reference genes for goosegrass. This study proposed to test the expression stability of 9 candidate reference genes in goosegrass in different tissues and developmental stages and under stress from three types of herbicide. The results show that for different developmental stages and organs (control), eukaryotic initiation factor 4 A (eIF-4) is the most stable reference gene. Chloroplast acetolactate synthase (ALS) is the most stable reference gene under glyphosate stress. Under glufosinate stress, eIF-4 is the best reference gene. Ubiquitin-conjugating enzyme (UCE) is the most stable reference gene under quizalofop-p-ethyl stress. The gene eIF-4 is the recommended reference gene for goosegrass under the stress of all three herbicides. Moreover, pairwise analysis showed that seven reference genes were sufficient to normalize the gene expression data under three herbicides treatment. This study provides a list of reliable reference genes for transcript normalization in goosegrass, which will facilitate resistance mechanism studies in this weed species.

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